254 BULLETIN 94. 



plasm has passed through into the center of the cell of the host. 

 From the free and smaller end of this oval cell a short protuber- 

 ance grows curving to one side usually rather close to the side of 

 the parent cell. Sometimes this branches quite soon in a dichoto- 

 mous manner and the two short cells curve in opposite directions. 

 If dichotomy does not occur at the beginning of the protuberance 

 another branch arises soon from the original cell or from the 

 branch. These protuberances become enlarged at a very short 

 distance from their origin forming oval cells. These in like manner 

 produce short branches, and the process continues until a botryose 

 or convoluted mass of cells is developed which eventually fills the 

 cell of the prothallium, and the elements of the botryoid body be- 

 come angular from mutual pressure. The wall now becomes 

 brown in color and the glomerule appears to be mature. 



In this condition if these hyphal masses are teased out from the 

 cell of the prothallium and kept on a glass slip in a small amount 

 of moisture germination soon takes place. Hyphal masses so 

 teased out from the prothallium and placed under the above con- 

 ditions at 5 P. M. on Feb. 22, 1894, and kept at the ordinary room 

 temperature during the night, the temperature falling somewhat 

 below that of the day (the temperature was 70 to 80 Fahr. , up 

 to midnight and fell 50 toward morning and rose to 66 at 9 A. M.). 

 At 9 A. M. Feb. 23d, the preparations were examined and the 

 mature hyphal masses were germinating. In some cases the germ 

 tubes were 5oo/u, to yoo/x long and all the protoplasm had moved 

 out in the distal half of the tube (fig. 42). In germination under 

 such circumstances a protuberance arises from one of the individ- 

 ual cells of the glomerule and extends soon into a tube the diame- 

 ter of which is about io/x. As the tube extends in length the pro- 

 toplasm gradually disappears from the parent cell and passes into 

 the tube. As the tube continues to elongate the protoplasm con- 

 tinues in the distal portion and the older portion of the tube be- 

 comes empty, nothing remaining but the wall. There appears to 

 be a wall at the junction of the tube with the parent cell, if so it 

 is formed after the protoplasm has passed into the tube.- When 

 the tube has become considerably elongated so that there is an 

 empty portion from 2oo/u, to 5oo/x in length there appear what seem 

 to be transverse septa, or it may be the remains of a portion of the 

 protoplasm situated in a thin transverse sheet in the tube. These 



