31 



ease was noticed among them by Messrs. Worthen and Cole, but it is 

 nevertheless possible that the wilt might have made its appearance. 

 No artificial means for destroying the gypsy moth had been undertaken 

 here. The number of egg clusters present at the time of my first visit 

 in the spring of 1910 was estimated by Mr. Worthen and myself at 

 about 550. 



Flacherie, which had been developed artificially by Mr. Cole, was 

 planted in this locality June 21, 1910, in the same manner as in the 

 place first described. The caterpillars were about ready for the fourth 

 molt, as in the first experiment. With the assistance of the division 

 agent the fresh clusters at this locality were estimated on Aug. 26, 

 1910, to be about 80. The microscopical examination of the first 5 

 clusters found shows : 



Unfertilized eggs, . 

 Eggs with dead embryos, 

 Eggs apparently alive, . 



Total, 



Unfertilized eggs, . 

 Eggs with dead embryos, 

 Eggs apparently alive, . 



Total, . 



Unfertilized eggs, . 

 Eggs with dead embryos, 

 Eggs apparently alive, . 



Total, 



Unfertilized eggs, . 

 Eggs with dead embryos, 

 Eggs apparently alive, . 



Total, 



Cluster 1. 



JQ \ = 6.67 per cent., or about 6.5 per cent. 

 . 154 =93.33 per cent., or about 93.5 per cent. 



. 165 eggs. 



Cluster 2. 



K f = 6.25 per cent., or about 6 per cent. 

 90 =93.75 per cent., or about 94 per cent. 



. 96 eggs. 



Cluster 3. 



2} 



- / = 7.09 per cent., or about 7 per cent. 



. 118 =92.91 per cent., or about 93 per cent. 



127 eggs. 



Cluster 4. 



3 \ 

 -.n } = 9.68 per cent., or cu^uim c/.^ pt v 



140 =90.32 per cent., or about 90.5 per 



about 9.5 per cent, 

 cent. 



155 eggs. 



Cluster 5. 



This cluster proved to be entirely without eggs, and it consisted only of a 

 mass of wool of about 1 square centimeter in size. The female moth, which 

 deposited this cluster, seems to have been absolutely sterile. It started 

 mechanically, it is true, to lay eggs, but of course it could deposit nothing 

 except the hairs from its abdomen. 



