390 AGRICULTURAL AND INDUSTRIAL BACTERIOLOGY 



in a flask containing glycerin bouillon, then killing by heat 

 and evaporating to about one tenth of the volume. Such 

 glycerin extract of the glanders bacillus (mallein) when 

 injected subcutaneously into animals suffering from glan- 

 ders will cause a rise in temperature usually beginning 

 between the fourth and eighth hour. An area usually the 

 size of a dinner plate about the site of the injection becomes 

 inflamed and swollen. As in tuberculosis the test is some- 

 times made by dropping purified mallein into the eye. In 

 animals which react the conjunctiva becomes inflamed, the 

 lids are swollen and a purulent discharge collects at the 

 inner corner and on the hair below. 



Transmission. The disease is transmitted readily from 

 one animal to another through infected foods, drinking 

 troughs and mangers, occasionally through wounds or skin 

 lesions. Veterinarians and horse men sometimes become 

 infected through the skin, rarely by inhalation. 



