1012 Bacterial Pyelonephritis. 



structure. The elongated thread-forms have club-shaped terminal 

 enlargements and spherical bud formations, also bulgings, excrescences 

 and often actual branching. 



Lieuaux & Zwaenopoel, also Hutyra have shown that this l)aeillus is closely 

 related to, if not identical with the B. pseudotuberculosis ovis, Preisz, which is 

 active in several other diseases. This would agree with Ernst's claim that the 

 B. renalis is to be classed in the group of the Corynebacteria to which the B. pseudo- 

 tuberculosis ovis belongs also. 



Cultivation. The Corynebacillus renalis is aerobic. It hardly 

 grows at room temperature, but kept on agar at the temperature of 

 the body cultures are formed which are fine, whitish, dry, sandy or 

 moist shining, or then dry, gray and scaly. In agar shake cultures 

 two zones form, one about 2 mm, the other 12 mm below the surface, 

 where the oxygen tension is most favorable for growth. On blood 

 serum tender, transparent whitish-gray dots grow which glisten like dew 

 drops ; in bouillon the cultures form a finely granular sediment. Ernst 

 has shown that the bacilli grow very well in neutral or faintly alkaline 

 urine, in urine agar or in iirine bouillon, and that in concentrated urine 

 agar a deposit of crystals forms around the stab which consists of triple 

 phosphates. Acids and indol are not produced. Certain cultural differ- 

 ences permit the assumption that there exist several slightly variable 

 strains. 



Pathogenicity. The injection of a pure culture under the 

 skin of mice or chickens produces no results (Enderlen, Ernst, 

 Masselin & Porcher), while in rabbits and guinea pigs either 

 a transitory swelling or an abscess develops (Enderlen, Kiinne- 

 mann, Ernst) or the animals are not affected at all (Lucet, 

 Masselin & Porcher). The same is observed after intraperi- 

 toneal or intrapulmonal inoculation of the culture (Enderlen, 

 Ernst). An intravenous injection produced purulent-cheesy 

 pneumonia in a rabbit (Ernst), intraocular inoculation often 

 a passing iritis (Enderlen, Kitt). Intravenous injection pre- 

 ceded by ligation of a ureter was followed in rabbits by a 

 process which was similar to pyelonephritis (Enderlen). In 

 sheep and in cattle subcutaneous injections are usually without 

 result or at most a temporary swelling develops at the point 

 of inoculation (Ernst), while intravenous injections of cultures 

 either remain likewise without result (Enderlen, Hoflich, 

 Kiinnemann) or Corynebacilli are eliminated with the urine 

 for several days or weeks without other disturbances (Hoflich, 

 Ernst). Intravesical injections in cows do not as a rule cause 

 any trouble (Hess, Enderlen, Hoflich, Kiinnemann, Ernst) or 

 they may exceptionally be followed by pyelonephritis (Masselin 

 & Porcher saw this in one case). A preliminary or simultaneous 

 irritation of, or injury to the mucosa of the bladder enables 

 the bacilli to localize in the bladder (Lienaux & Zw^aenopoel, 

 Ernst), and according to Lienaux and Zwaenopoel a pyelone- 

 phritis may even follow. 



Schmidt claims to have found the Corynebacillus renalis 

 also in a colt with bacterial pyelonephritis. 



