488 BOARD OF AGRICULTURE. [Pub. Doc. 



One hundred and thirty-nine were evident cases of disease, 

 and were ordered killed. 



Forty-eight were clearly free from disease, and were or- 

 dered released. 



Fifty-two were doubtful cases, from which guinea pigs 

 were inoculated. 



In 27 instances the guinea pigs developed glanders, and 

 the horses were destroyed. 



In 25 cases the guinea pigs did not develop glanders. Of 

 these, 21 horses were released and 4 were killed, having de- 

 veloped such marked clinical evidences of glanders while 

 awaiting the results of the guinea-pig test as to render an- 

 other test unnecessary. In one instance it was necessary to 

 inoculate guinea pigs three times from the nasal discharge of 

 a horse suspected of having glanders and farcy before they 

 developed the disease, there not being enough germs of 

 glanders present in the material taken the first and second 

 times to infect these little animals ; yet the horse was so 

 suspicious that the Board did not feel that it ought to be re- 

 leased on the negative tests. The final results proved the 

 suspicion to be well founded. In one or two other cases a 

 second inoculation was necessary, but generally the test is 

 decisive on a single trial. 



Two horses were killed on a mallein test, and 1 released. 



One case given as negative was not reported to the Board 

 until the owner had disposed of it, and there is no positive 

 proof that it had glanders ; on the doubt, it is entered as 

 negative. 



A case reported as positive from Great Barrington is 

 doubtful ; the horse died in quarantine, and there was not a 

 careful autopsy made upon it. 



The 375 remaining cases mentioned were killed with the 

 owners' consent, except 159 reported from the city of Bos- 

 ton, where the board of health had full charge of glanders, 

 and 3 or 4 that died in quarantine, leaving IIG killed with 

 the owners' consent outside of the city, except the few which 

 died of the disease. Many of these were killed upon the 

 advice of veterinarians, and in a great number of cases were 

 reported to the Board as killed with the consent of the 

 owner. 



