STAINING METHODS 4$ 



time, and filtering the solution through a wet filter-paper. 

 Gentian violet can be replaced by 11 c.c. of saturated 

 alcoholic solution of fuchsin or methyl violet. 



The prepared stain should not be kept longer than two 

 weeks. 



Carbol -Gentian Violet : 



Saturated alcoholic solution of gentian 



violet . . . . . . . . . . 10 c.c. 



Five per cent, phenol ... .. .. 100 c.c. 



ZiehVs Carbol-Fuchsin : 



Fuchsin . . . . . . . . 1 gramme. 



Phenol . . . . . . . . 5 grammes. 



Absolute alcohol . . . . . . 10 c.c. 



Distilled water 100 c.c. 



The fuchsin is dissolved in the alcohol, and the phenol, 

 previously dissolved in the water, is then added. 



For ordinary cover-glass preparations this solution is 

 diluted with water in the proportion of 1 : 6. 



In staining for tubercle bacilli, it must be remembered 

 that this dye sometimes loses its staining properties with 

 age, and it shouldjbe tested on a sputum known to contain 

 the bacillus in large numbers. 



Lo filer's Methylene Blue : 



Saturated alcoholic solution of methy- 



lene blue . . . . . . . . 30 c.c. 



Caustic potash solution (1 : 10,000) .. 100 



This solution keeps well. 



Kuhne's Carbol- Methylene Blue : 



Methylene blue . . . . . . 1-5 grammes 



Absolute alcohol . . . . . . 10 c.c. 



Five per cent, aqueous solution of 



phenol . . . . . . . . 100 c.c. 



Nicolle's Garbol-Thionine Blue : 



Saturated solution of thionine blue in 



90 per cent, alcohol . . . . . . 10 c.c. 



One per cent, aqueous solution of 



phenol . . . . . . . . 100 



Eosin : | to 1 per cent, solution in water or alcohol. 



