THE BLASTOMYCETES 167 



Bottcher chamber is -painted with sterile melted vaseline, 

 and on this the gelatin- coated cover-glass, infected 

 surface downwards, is placed and pressed down, so that 

 the chamber is completely sealed. The edge is painted 

 with a melted mixture of 1 part of wax and 2 parts of 

 vaseline, to prevent the cover-glass moving. The prepara- 

 tion is examined, the position of isolated cells is marked, 

 and the chamber incubated at room -temperature till the 

 colonies have become sufficiently large to be transferred 

 to liquid media. With a Bottcher chamber with a ring 

 of 30 millimetres diameter, twenty to thirty cells is a 

 convenient number to have in the gelatin mixture. The 

 method needs some practice to obtain results. 



Torulse (vide supra). The Torulce produce little or no 

 alcohol in saccharine liquids. Saccharomyces rosaceus, 

 niger, and albus are met with in air, and form coral pink, 

 sooty black, and white growths respectively on potato 

 and bread. 



Yeasts in Sour Milk. Herschell and Emerson agree 

 with the use of a yeast in the preparation of Bulgarian 

 soured milk for therapeutic purposes, not only to improve 

 the flavour and to attack the milk-sugar after lactic 

 acid has been formed, but also to inhibit the overgrowth 

 of pathogenic and putrefactive bacteria. It is not un- 

 usual for the yeast to get the upper hand, and carry on 

 the fermentation much too far. 



Pathogenic Yeasts. Blastomycetic dermatitis (blasto- 

 mycosis) is sometimes seen in man. The disease may 

 become generalised, and sometimes ends fatally. Yeasts 

 have also been obtained from tumours, and some con- 

 nection between yeasts and malignant tumours has been 

 suggested. An outbreak of sore throat at Lincoln was 

 thought by Klein to be caused by a yeast found in a 

 throat. The yeast produced swelling of the throat, 

 fauces, and larynx in rabbits. 



CHAPTER XVI 

 THE HYPHOMYCETES 



THE moulds or Hyphomycetes are multicellular organisms 

 composed of filaments (hyphae), which, when interlacing, 

 form a mycelium. A mycelium may form a hard woody 



