Survey gauging station (#12340000) at Bonner at river mile 7.9 to examine potential 

 relationships between discharge and fish movement. • • / 



The incubation period for RBT was calculated from known water temperatures and a 

 median spawning date of RBT in Monture Creek, using a 340 temperature-unit value 

 (Ron Snyder, Arlee Hatchery, personal communication). Emergence was estimated at 

 approximately three weeks post-hatch. The University of Montana, Trout and Wild 

 Salmon Genetics Laboratory, Missoula, Montana using DNA (PINES) analysis of anal 

 fin clips, assessed likely genetic status of the twenty 2005 individuals. 



Relocation data was spatially located using GPS receivers (Garmin III), 1:24,000 

 maps, recognizable landmarks or a combination of these techniques. Relocations were 

 converted to latitude and longitude and entered in degree decimals to Arc View GIS point 

 coverage with all relational data attached using EXCEL databases and converted to river 

 miles. Within tributaries, movements were expressed as the distance upstream from the 

 mouth. 



Following 

 identification of 



primary spawning 

 sites by telemetered 

 RBT and to determine 

 infection severity, 

 sentinel cages - each 

 with 50 young-of-the- 

 year RBT were 

 placed central to 

 spawning locations in 

 July 2005. We also 

 placed sentinel cages 

 at all other known 

 spawning basin-fed 

 tributaries in areas of 

 known RBT 



At the end 



y^^'W/^: 



Blackfoot River 



'«-' 



w, 



.fw 



•«^f>., 











' Clark Fork River 



Bitterroot River 



Figure 50. Summary of all RBT telemetry locations in 2004 and 2005. 



spawning. 



of the holding period, all surviving fish were sacrificed and sent to the Washington State 

 University Animal Disease Diagnostic Laboratory at Pullman, WA. At the lab, the heads 

 were histologically examined using the MacConnell-Baldwin histological grading scale, 

 which ranks infection intensity from (absent) to 5 (severe) (Baldwin et al. 2000) (See 

 Procedures section of sentinel cage testing). 



Although not included in this preliminary report, we also measured the physical 

 characteristics of all RBT spawning areas where sentinel cages were located. These 

 included geomorphic surveys (Rosgen 2002), McNeil substrate core samples (Results 

 Part IV), water chemistry (PH, conductivity and total dissolved solids) testing and 

 measurements of summer water temperatures. 



Preliminary telemetry results 



n 



65 



