IN I KODUCTION. 



1 1 m.i this, well illustrated in the group Cirripedia, 

 which forced Darwin slowly bul surely to frame and 

 enunciate his evolut ion by pothesis. 



■■ As plant after plant has passed under my obsi rva 

 lion, I ha i been greatly impressed, no1 only with the 

 averagi similarity in development thai each Bhows, bul 

 even more with the constanl tendency there is for indi- 

 viduals to vary from that average either in under or 

 over development, it may be only of some part or ana 

 ot of some large organ. As illustrations on a somewhat 

 large scale, I may refer to the number, position on the 

 st.ni, and size of leaves, a Line of inquiry which has been 

 entirely overlooked by systematists, but which can afford 



charai ■> iderable value. Thus Hedychium gard 



mum, when well grown and not overcrowded in a 

 hot-house, semis up flowering shoots which bear on the 

 average 13 lamina-producing leaves, beside one or two 

 basal scales. II. coronarium hears 21, while the hybrid 

 //. sadlerianum bears 17. But not unfrequently from 

 overcrowding, lack of light and nourishment, or other 

 unfavorable surroundings, the number in each may be 

 lerably reduced. Conversely, when very favorable 

 vegetative conditions occur, these are accompanied with 

 er luxuriani e. 



"A shoot of Saxifraga aizoon, with freedom for 

 growth, produces annually 23 to 26 leaves; >'. geum, 

 40 to 45 ; and their hybrid, 8. andrewsii, 30 to 33. 



"During the autumn of 1890 I happened to go over 

 a large bed of sunflowers, and, in by far the greater num- 

 ber, 27 to 28 leaves were formed between the cotyledons 

 and terminal capitulum. A few in tructive cases of 

 variability from the average were noted. The bed was 

 one which sloped to the sun and some plants at the bai ■ 

 that were slightly overshadowed by trees had bi en starved 

 in their light and moisture supply. Their leaves were 

 reduced to 20 or 21. < >n the other hand, one in a favor- 

 able situation produced 31 leaves. 



"But minute changes are correlated with these 

 grosser variation-, such as an increase or decrease in the 

 stomata over a given area or in the length and number 

 of hairs, etc. In the choice of material, therefore, for 

 hybrid investigation one should either he acquainted 

 with the parent individuals and the conditions under 

 which they were grown or try to choose an average 

 men of each for study. 



2. Limit of Variability-. 



"A wide field of patient and laborious work is open 

 in the direction of ascertaining how far the individuals of 

 a species may differ microscopically without losing spe 

 eilie identity. As yet this field may be said to be un- 

 trodden. The contributions that have recently been 

 made (Bot. Central., lid. \iv, xt.vi) by Schumann are 

 exactly on the lines desiderated and form a valuable 

 study in tissue variability, but if we are to get an exact 

 estimate alike of Bpecies and hybrid production the 

 knowledge must be forthcoming. Thus Lapagi ria rosea 

 . a parent form which I have chosen for pretty exhaus- 

 i. .e description, and though 1 have tried to select mate- 

 rial from what I regard as an average strain, tin's may 

 still differ from the parent plant usi d, as several varieties 

 are known to be in cultivation. This may partially es 

 plain why it is that hybrids at times exhibit a slight 



divergence toward one parent. Again, 1 -hall have to 



it some length to the remarkable changi • 

 exhibited by the flowers of Dianthus ■ rom white 



on first opening to rich crimson or crimson-purple on 

 fading. The one par, ni. I>. alpinus, . any 



trace of such floral change, but among the numerous 

 varieties of P. barbatus in cultivation one exhibits the 

 above peculiarity in an equally or even more striking 



manner. 



"Now, every varietal form inherits certain 



peculiarii ies, and also the point tamp it as 



a variety, so that one would err in comparing the ordi- 

 nary species with the hybrid. But the I that 

 ies are often im onstant in their varietal details, and 

 do not hand these down in all cases so steadily 

 marked species, are n asons i i our giving a certain lati- 

 tude in comparison with the hybrid, but equal 

 reasons for our desiring an exact knowledge of how far 

 a specific form may vary. 



3. Comparison of Similar Paris. 



" In my earlier investigations it was somen. 

 found that a certain part or organ of a hybrid did not 

 exhibit intermediate blending of the structure of both 

 parents, but a decided leaning to one. This was at 

 regarded as an instance of variation from a brid- 



itv, but more careful and exhaustive comparison sh 

 that the apparently exceptional conditions arose from 

 choice of material that did not agree in age, position, or 

 opportunities for growth. Thus 1 stated in the ' 

 deners' Chronicle' (April 1890) that while Saxifraga 

 aizoon bad many stomata on its upper leaf surface and 

 S. geum had none, S. an resembled the la1 



this respect. Now, I had expected to a the 



leaf chosen from the hybrid, which wa 

 of an annual shout, those of the parents being from the 

 upper parts of shoots. On returning to the matter more 

 recently, it was found that the closely intermediate 

 character of the hybrid was established when leaves of 

 the same relative position and age were chosen. Thus, 

 since S. aizoon produces on the average 2 i annually, 



the hybrid 32, and S. geum 40, if the tenth leaf from the 

 base be chosen in the first, we should select the four- 

 teenth in the hybrid and the eighteenth in the other 

 parent. The same principle of judi ection of 



material must be applied not only in dealing with large 

 organs but also in minuter details, such as bundli 



nts, matrix cell-, and sclerenchy ma, a- well as starch 



grains, chloroplasts, and other cell products. 



■I. Available Limit FOR CoMPABISON ok Pabehts with their 

 llviiuin Pbooent. 



"During the last decade problems bearing on the 

 relative potency of the male and female elements in the 

 development of an organism have been greatly discussed, 

 The present investigation not only th] it light on 



these, but will enable us to compare more accurately than 

 hitherto the capability s of each sex element. It is mani- 

 fest, however, that when a hybrid is the p 

 parents that are widely divergent in histological details 

 the comparison will be easy, hut when we attempt to 

 compare a hybrid with two parents which are regarded 

 as species, but whose chief specific differeno - are those 

 of eoloi ing and size, it is almost or quite impos ible to 



