HIPPEASTRUM. 



43 



mid-intermediate in the reaction with iodine. In the 

 polarization and temperature reai tiona it is closer to the 

 pollen parent, and in the gentian-violet and safranin 

 reactions closer to the seed parent 



Table A.; shows the reaction-intensities in pet 

 oi total starch gelati it definite intervals 



(minutes). 



Velocity-reaction Cl r\ 



This section treats of the velocity-reaction curves of 

 the starches <>t' Hippeastrum ossultan, II. pyrrha, and 

 II. ossvitm-pyrrha, showing the quantitative differences 

 in the beha\ ior inward different reagents at definite time 

 intervals. (Charts D 13 to D 63.) 



The conspicuous features of these charts do not differ 

 in many respects from those of the preceding set. 



(1) The curves of all three starches are in all of the 

 r< ai hions close ami. on the whole, about the same as 

 regards the extent of separation as m the first set, in 

 smiir reactions there being a little more separation and 

 in others less. Iu most of the reactions there is a ten- 

 deney fur a slightly higher reactivity than in the //. 

 titan-cleonia set. Many of the reactions are so slow 

 that there is no important if any differentiation, as in 

 those with potassium sulphide, sodium sulphide, calcium 

 nitrate, uranium nitrate, strontium nitrate, cobalt ni- 

 trate, copper nitrate, cupric chloride, barium chloride, 

 and mercuric chloride. 



(2) Omitting these very slow reactions, the curve 

 of //. ossultan is in the remaining 11 reactions higher 

 than the corresponding curve of the other parent in 

 the reactions with chloral hydrate, chromic acid, nitric 

 acid, potassium iodide, potassium sulphocyanate, sodium 

 hydroxide, and sodium salicylate; and lower in those with 

 pyrogallic acid, sulphuric acid, hydrochloric acid, and 

 potassium hydroxide. 



(3) The curves of the hybrid hear varying relations 

 to the parental curves, with very little tendency to same- 

 ness in relation to the seed parent and none to the 

 pollen parent; with little tendency to intermediateness 

 or to being the lowest of the three curves; with a marked 

 tendency to be the highest of the three; and with a ten- 

 dency to sameness as both parents in the reactions that 

 take place with marked slowness. (See the following 

 section.) 



(-1) An early period of comparatively high resistance 

 i- Doticed especially in the reactions with chloral hydrate, 

 chromic acid, nitric acid, hydrochloric acid, and potas- 

 sium sulphocyanate; the opposite with potassium hy- 

 droxide and sodium salicylate. 



(5) The best period for the differentiation of the 

 three starches is in case of the very slow reactions above 

 referred to at the end of the 60 minutes, but in some of 

 them even at this time there is very little or no differ- 

 ence. The curves appear to be best separated at 5 min- 

 utes in the reactions with sulphuric acid, potassium hy- 

 droxide, and sodium salicylate; at 15 minutes with 

 chloral hydrate, chromic acid, pyrogallic acid, and so- 

 dium hydroxide; at 30 minutes with nitric acid, hydro- 

 chloric acid, and potassium sulphocyanate. 



Reaction-intensities of the Hybrid. 



This section treats of the reaction-intensities of the 

 hybrid as regards sameness, intorniodiateness, excess, and 

 deficit in relation to the parents. (Table A 3 and Charts 

 D43 toDG3.) 



The reactivities of the hybrid are the same as those 

 of the seed parent with sulphuric acid, sodium sulphide, 

 and uranium nitrate; the same as those of the pollen 

 parent in none; the same as those of both parents with 

 potassium sulphide, calcium nitrate, strontium nitrate, 

 cobalt nitrate, copper nitrate, cupric chloride, barium 



chloride, ami mi rcuric i > with 



iodine, chloral hydt lium hydi I a the 



iir-t being mid-intermed ate and in the I tearer 



the seed parent) ; highest with polarization, gentiai 

 let, safranin, temperature, chromic acid, u 

 pyrogallic ai id, hydrochloric a< id, potas ium hydn 

 le, and pota ium .- ulphocyanate ( 

 being closer to the Beed parent and in fivi 

 to the pollen parent) ; and the lowest with 6odiu 

 late, it being in these nearer the pollen parent. 



The follow ing i a i iimmarj of ti- 

 tles: Same as seed parent, 



same as both parents, 9; int b, 11; 



lowest, 1. 



In not a single reaction is there saiuem 

 to the pollen parent, and ti ;er influence of the 



Beed parent on the propi 1 1 i tie hybrid is quite 



marked. Intermediateness is rather ■ 

 dency to the lowest reactivit} m and a 



tendency to the highest reactivity very n 



( lOMPOSITE Cl RVES OF THE REAC] tON-INTl STSI1 



This section treats of composite curves of the reac- 

 tion-intensities showing the differentiation of the 

 starches of Hippeastrum ossultan,, II. pyrrha, and II. 

 ossultan-pyrrha. (Chart E3.) 



Among the conspicuous features of this chart are: 



(1) The remarkable closeness of all three cui 

 the differences for the ificant or 

 actually Tailing within the limits of en 



showing an extreme botanical i 



and extremely little variance of the h; i u the 



parents. The only reactions in whicl ts are 



readily differentiated are those with ioi ntian 



violet, safranin, temperature, chromic acid, and sodium 



salicylate, and even in these the i 



exi i ption of a minor degree. 



(2) In tin- curve of //. ossultan compared with that 

 of //. pyrrha the reactivities are si. own to be distinctly 

 higher in the reactions with gentian vi anin, 

 chromic acid, and sodium salicylate, and lower with 

 polarization, iodine, and temperature. In the other in- 

 stances the differences are unimportant or even negligible 

 excepting in so far as they tend to indicate a . 

 slightly higher reactivity of //. ossultan. 



(3) In II. ossultan the very high reactions with 

 polarization, chromic acid, sulphuric acid, and sodium 

 salicylate, the moderate reactions with iodine. 

 gentian violet, and pyrogall . the low 



with temperature, nitric acid, hydrochloric acid, 

 shim hydroxide, and potassium sulphocyanate; an 

 very low reactions with chloral hydrate, potassium iodide, 

 potassium sulphite, sodium hydroxide, a dium 

 calcium nitrate, uranium nitrate, strontium nitrate, 

 ball nitrate, copper nitrate, cupric chloride, barium 

 chloride, and mercuric chloride. 



(4) In H. pyrrha the very high reactions with polari- 

 zation, sulphuric acid, and sodium salicylate; the high 

 reactions with chromic acid, the moderate i with 

 iodine, gentian violet, safranin and pyrogallic ace . 

 low reactions with temperature, nitric acid, hydrochloric 

 acid, potassium hydroxide, potassium sulph 



the very low reactions with chloral hydrate, potassium 

 iodide, potassium sulphide, sodium hydi lium 



sulphide, calcium nitrate, uranium nitrat tium 



nitrate, cobalt nitrate, copper nitrate, cupric 

 barium chloride, and mercuric chloride. 



(5) In the hybrid the very high >lar- 

 ization, chromic acid, sulphuric acid, pyrogallic acid. 

 sodium salicylate; the moderate reactions with iodine, 

 gentian violet, safranin, temperature, and hydrochloric 



