II KMANTIITTS. 



47 



or deficit of parental extremes is more common than 

 intermediateness, for in 21 reactions the hybrids were 

 higher than those of either parent and in 9 lower than 

 those of either parent. In case of all three hybrids the 

 seed parent seems to be the more poteni in influencing 

 the characters of the starch, this potency being the most 

 marked in //. ossultan-pyrrha and least marked in //. 

 daones-zephyr. 



. r >. CoMPABISONS t'l THE STAR! HES 01 II MANTH1 S 

 k \ I II 1:1:1 X i:, H. MAGNIFICUS, AND II. ANDROMEDA. 



In histologic characteristics, in polariscopic figure . 

 in the reactions with selenite, in the reactions with 

 iodine, and in the qualitative reactions with the various 

 chemical reagents it will he noted that the parent starches 

 not only exhibit properties in common in variable de 

 grees of development, but also individualities which col- 

 lectively serve to distinguish them. 



The starch grains of Hosmanthus magnificus contain 

 proportionately a larger number of aggregates; there 

 are compound grains that are not found in //. katherince; 

 and the grains tend to more irregularity, to more breadth 

 in relation to length, and to rounded ends. The hilum 

 is more distinct and more frequently fissured, but the 

 eccentricity is about the same; the lamellae are less 

 distinct; and the size is larger, with a tendency to 

 broadness. In polariscopic figure and reactions with 

 selenite there are various differences. The grains of the 

 hybrid II. andromeda are in form in general closer 

 to those of II. katherince, and in certain respects closer 

 to those of the other parent. They are more irregular 

 than those of either parent, and there are compound 

 grains like those found in //. magnificus, but they are 

 less numerous. In the character of the hilum and in size 

 they are closer to those of II. katherince, but in lamelhe 

 there does not appear to be a definite leaning toward one 

 or the other parent. In the polariscopic figure and 

 appearances with selenite the grains are closer to //. 

 katherince, and the same is true in regard to their quali- 

 tative behavior with iodine. In the qualitative reac- 

 tions with chloral hydrate, nitric acid, potassium iodide, 

 potassium sulphocyanate, and sodium salicylate the 

 grains show a close relationship to those of II. katherince, 

 except in the case of a few grains in each reaction which 

 show a corresponding relationship to //. magnificus. On 

 the whole, the relationship is very close to //. katherince. 



Reaction-intensities Expressed by Light, Color, and Tempera- 

 ture Reactions. 

 Polarization: 



H. katheriint, high to very high, value 75. 



H. magnificus, very high, much higher than H. katherince, value 90. 

 H. andromeda, high to very high, higher than II. katherine, 

 value 82. 

 Iodine: 



H. katherinffi, moderate to light, value 45. 

 H. magnificus, moderate, deeper than II katherince, value 50. 

 II. andromeda, moderate to deep, a little deeper than II katherince, 

 value 47. 

 Gentian violet : 



H. kathcrina*. moderate to deep, value 60. 



II. magnificus, moderate to deep; not so deep as II. katherince, 



value 55. 

 H. andromeda, moderate to deep, slightly lighter than II. katherina-, 

 value 58. 

 Safranin: 



H. katherince, moderate to deep, value (50. 



H. magnificus, moderate to deep, the same as II. katherince, value 60 

 H. andromeda, moderate to deep, lighter than in the parent-stock, 

 value 58. 

 Temperature: 



H. katherina?, majority at 7'.) to 81°. all at 82 to 84°, mean S3". 



H. magnificus, majority at 77 to 77.5°, all at 78 to 79°. mcai 



H. andromeda, majority at 75.5 to 80°, all at 81 to 82°, mean 81 5° 



The reactivities of H. katherina are lower than 

 those of H. magnificus in the reactions with polarization, 



iodine, and temperature; higher with iolet; and 



ame with safranin. The reactivities of the hybrid 

 are intermediate in the reactions with polarization, io- 

 dine, gentian violet, ami temperature; and lower than 



of the parents with safranin. With the e 

 tion of the last and the temperature reaction the 

 tionship of the hybrid i- practically exactly mid-inter- 

 mediate, and in the temperature reaction it is closer to 

 //. katherince. 



Table \ 5 reaction-intensities in per 



ages of total starch gelatinized at definite inte 

 ( minutes) : 



\ ELOCITY-R1 



Thi 



of the starches of Tlccmanthus katherince, II. n 

 and //. andromeda, shoi 



in the behavior toward differenl reagents at definite time- 

 intervals. (Chart D8,-) to I) L05.) 



The most conspicuous features of these charts are: 



(1) The individualities of each chart in relation to 

 the reagent, except in 



30 slow and the figures gi - to be within the limits 



of error. In the charts in which the i are other- 



wise than very slow tie- thret vary in their close- 



ness to one another within wide limits. Thus, in the 

 reactions with chromic acid and sulphuric acid all three 

 curves keep close together throughout tie' 60 mi 

 but the charts are readily distinguishable from each 

 other, especially at the 15- and 30-minute period-, at 

 which tilth ier in the sulphuric- 



acid chart. The curves for chloral hydrate, nitric acid, 

 and hydrochloric acid show a tendency during the prog- 

 ress of the reactions to divergence, in all three charts 

 the curves of the hybrid being intermediate, hut in two 

 closer to the curve of //. katherina;. The chart for 

 -odium salicylate stands isolated, owing lly to 



the relatively high reactivities of the hybrid and II. 

 katherince during the first 5 minutes. In all of the 

 chart.-, in which the three curves are sufficiently separated 

 to make satisfactory determinations, the curve of the 

 hybrid, with the exception of a few instate - defi- 



nitelv to intermediateness. 



(2) The curves of If. ma in the reactions 

 with chloral hydrate, pyrogallic 



tassium hydroxide, potassium sulphocyanate, sodium 

 salicylate, and sodium hydroxide, in all of which the 

 reactivities are sufficiently marked to bring out positive 

 differences in reacti . are the highest except- 



ing in two cases (chloral hydrate and sodium salicylate), 

 in both of which the curves of II. kaiherinw are the high- 

 est — a curious reversal of position. In all of the charts 

 in which positive differences have been brought oul 

 curve of the hybrid tends to be cl E II. kath- 



erina irrespective of ition of the latter in relation 



to the curve of //. magnij 



(3) The curves of the hybrid, except in the reactions 

 in which all three curves arc essentially the same, tend to 

 be the same as those of the seed parent or of some degree 

 of intermediateness. Tn the latter group there is an 

 obvious tendency to mid-intermediateness oi to the 

 parent. 



Reaction-intensities of the Hybrid. 



The following section treats of the reaction-intensi- 

 fies of the hybrid as regards sameness, intermediate 

 excess, and deficit, in relation to the parents. (Table A 5 

 Charts D 85 to D 105.) 



The reactivities of the hybrid are the same as those 

 of the seed parent in the pyrogallic. acid, potassium 

 iodide, potassium sulphocyanate, sodium hydroxide, so- 

 dium sulphide, calcium. nitrate, uranium nitrate, and 



