90 



HISTOLOGIC PROPERTIES AND REACTIONS. 



Temporal me: 



N. emperor, in majority at 69 u>7l rj , iaa!lat74 to 75. 5°, mean 74.53°. 

 riandrus albus, in majority at 70 to 71°, in all at 73 to 75°, 

 i.i 

 N. j. t. bennett poe, in majority at 04 to 04. b°, in all at 09 to 71°, 

 mean 70°. 



The reactivity of A T . emperor is higher than that of 

 the other parent in the polarization, gentian violet, and 

 safranin reaction; and lower in the iodine and tempera- 

 ture reactions. The reactivity of the hybrid is the same 

 or practically the same as that of A 7 , emperor in the 

 polarization and iodine reactions; and the highest of the 

 three in the gentian violet, safranin, and temperature 

 reactions. There is no instance of intermediateness, and 

 in certain respects the starch of the hybrid is nearer to one 

 parent and in others to the other parent. 



Table A "J 1 shows the reaction-intensities in percent- 

 of total starch gelatinized at definite intervals 

 (minutes) : 



Table A 24. 



Velocity-reaction Curves. 



This section treats of the velocity-reaction curves of 

 tarcb.es of Narcissus emperor, N. triandrus albus, 

 and N. j. t. bennett poe, showing the quantitative differ- 

 ences in the behavior toward different reagents at definite 

 time-intervals. (Charts I) 31 1 to D 34G.) 



The most conspicuous features of these charts are : 



(1) The correspondence in the three curves in all 

 of the reactions, and the general tendency to a high to 

 moderate reactivity. 



(?) The varying relationships of the parental curves 

 to each other and to the curve of the hybrid in the dif- 

 ferent reactions. 



('■'<) The curve of X. emperor is practically the same 

 ;is thai i.i' the oilier parent in the py rogallic-acid reac- 

 tion and higher in the reactions with chloral hydrate, 

 chromic acid, pyrogallic acid, and sulphuric acid, the 

 most marked difference being noted in the pyxogallic- 

 acid reaction and the least in the quick sulphuric-acid 

 reaction. 



I l i The curve of the hybrid is the same as that of 

 N. emperor in the very rapid sulphuric-acid reaction; 

 practically the same in that with chloral hydrate; nearly 

 the same in that with pyrogallic acid: intermediate in 



none; and the highest of the three in those with chromic 

 acid ami pyrogallic acid. In all of the reactions the 

 hybrid shows a higher reactivity than either parent. 



(5) A tendency to an early period of resistance fol- 

 lowed by a comparatively rapid reactivity is seen in all 

 three starches in the reaction with chromic acid, and in 

 the two parental starches in that with pyTogallic acid. 

 The earliest period at which the three curves are best 

 separated for differential purpose is in the sulphuric-acid 

 reaction at the beginning; in those with chloral hydrate, 

 chromic acid, pyrogallic acid, and nitric acid at 15 

 minutes. 



Eeaction-intkxsities of the Hybrid. 



This section treats of the reaction-intensities of the 

 hybrid as regards sameness, intermediateness, excess, and 

 deficit in relation to the parents. (Table A 24 and 

 Charts D 341 to D 34G.) 



The reactivities of the hybrid are the same as those 

 of the seed parent in the polarization and iodine reac- 

 tions; the same as those of the pollen parent in none; 

 the same as those of both parents in none; intermediate 

 in none; highest in those with gentian violet, safranin, 

 temperature, chloral hydrate, chromic acid, pyrogallic 

 acid, nitric acid, and sulphuric acid (in six being closer 

 to those of the seed parent, and in two closer to those of 

 the pollen parent). 



The following is a summary of the reaction-intensi- 

 ties (10 reactions): Same as seed parent, 2; same as 

 poller parent, 0; same as both parents, 0; intermediate, 

 ; highest, 8 ; lowest, 0. 



The seed parent seems to have almost entirely con- 

 trolled the development of the properties of the hybrid, 

 inasmuch as in 10 out of the 12 reactions there is same- 

 ness or nearness in relation to this parent. Another 

 equally striking feature is the almost universal tendency 

 for the reactivity of the hybrid to exceed parental 

 extremes. 



Composite Curves of Reactiox-ixtexsities. 



This section treats of the composite curves of the reac- 

 tion-intensities, showing the differentiation of the 

 starches of Xarcissus emperor, X. triandrus albus, and 

 N. j. t. bennett poe. (Chart E 24.) 



The most conspicuous features of this chart are: 



(1) The close correspondence in the courses and 

 closeness of the curves throughout the chart. 



(2) In N. emperor in comparison with A", triandrus 

 albus the higher reactions with polarization, gentian vio- 

 let, safranin, chloral hydrate, and chromic acid; the 

 lower reactions with iodine and nitric acid ; and the same 

 or practically the same reactions with temperature, pyro- 

 gallic acid, and sulphuric acid. 



(3) In N. emperor the very high reaction with sul- 

 phuric acid; the high reactions with polarization and 

 iodine; the moderate reactions with gentian violet, safra- 

 nin, chromic acid, and pyrogallic acid ; the low reactions 

 with temperature and nitric acid ; and the very low reac- 

 tion with chloral hydrate. 



(4) In N. triandrus albus the very high reaction 

 with sulphuric acid; the high reaction with iodine; the 

 moderate reactions with polarization, safranin, chromic 

 acid, and pyrogallic acid; the low reactions with gentian 



