132 



HISTOLOGIC PROPERTIES AND REACTIONS. 



Gentian violet: 



M. vexillaria, moderate, value 50. 



M. rcezlii, moderate to deep, deeper than in M. vexillaria. value 55. 

 M. bleuana, moderate to deep, lighter than in M. vexillaria, 

 value 17. 

 Safranin: 



M. vexillaria, moderate to moderately deep, valu i 

 M. rcezlii, modi i '" M. vex- 



illaria, valu. I i 

 M. hi. nana, moderate t<. moderately deep, the same aa in M. vex- 

 illaria, value 55. 

 Temperature : 



M. vexillaria. in the majority at 70 to 71°, in all but rare grains at 



;V\ mean 73.5°. 

 M. rcezlii, in the majority at 71 to 76°, in all but rare grains at 



7ti to 77". mean 76.5°. 

 M. hi. nana, in the ii 69 to 71°, in all but rare grains at 



72 to 7-1°, mean 73°. 



M. vexillaria shows a higher reactivity than the 



other parenl in the polarization, iodine, and temperature 



,„,-. and ;i lower reai tivity in the gentian-violet and 



ons. The hybrid has the highest re^^_ 



i the three in the polarization and 1|,1 ' ll l"'. r .\ niIV ,.', IC „ 



. the lowest reactivity in the gentjajg^g^ reactions, 



and the same or practically tl,. ; gr£ reactivities as M . 



; ''"' "' tU ! : ';"';"" a V.„ aafranin reactions. In all 

 five reactions the hj^ ls either the same as or closer 

 to .1/. vextllaj J 



T>.,1,1. -•■>• '"• . . 



■lyi.nf A43 shows the reaction-intensities in percent- 

 of total starch gelatinized at definite intervals 

 (minutes I. 



Velocity-reaction Curves. 

 This section treats of the velocity-reaction curves of 

 the .-tan lies of Miltonia vexillaria, M. rcezlii, and ill. 

 ma, showing the quantitative differences in the be- 

 or toward different reagents at definite time-inter- 

 nals. (Charts D 595 to D 609.) 



Among the conspicuous features of these charts are: 

 I loseness ami correspondence of the curves in each 

 of the reactions. The reactions with nitric acid, sul- 

 phuric acid, hydrochloric acid, and potassium hydroxide 

 occur with such rapidity that there is practically no 

 differentiation. The curve of .1/. vexillaria is higher 

 than the curve of the other parent in the reactions with 

 chloral hydrate, chromic acid, pyrogallic acid, potassium 

 iodide, potas-iuin -ulphocyanatc, potassium sulphide, so- 

 dium hydroxide, -odium sulphide, sodium salicylate, cal- 

 cium nitrate, uranium nitrate, strontium nitrate, copper 

 nitrate, i npric chloride and mercuric chloride; and lower 

 with cobaH nitrate and barium chloride The hybrid, 

 while hearing varying relations to one or the other or both 

 parents as regards sameness, intermediateness, excess, 

 and deficit in reactivities, shows a remarkable inclination 

 to an almo.-t universally higher reactivity than either of 

 the parents, and, moreover, a similar inclination to the 

 d parent; in only 2 of the 26 reactions is there a mani- 

 |i aning toward the pollen parent. An early period of 

 igh resistance followed by rapid to moderate gelatiniza- 

 in.ii is entirel] ab eni from this set of reactions. The 

 earliesi period during the 60 minutes that is best for the 

 differentiation of the three starches is for chromic acid, 

 potassium iod num sulphide, potassium snlpho- 



ate, sodium hydroxide, sodium sulphide, sodium 

 salicylate, uranium nitrate, strontium nitrate, cobalt ni- 

 trate, copper nitrate, and cupric chloride at 5 minutes; 

 calcium nitrate at L5 minutes; chloral hydrate, pyro- 

 gallic acid, barium chloride, and mercuric i hloride a1 30 

 minutes. The reactions with nitric acid, sulphuric acid, 

 hydrochloric acid, and potassium hydroxide are too fast 

 for differentiation of the starches. 



REACTIO! INTENSITIES 01 THE II Yl'.KID. 

 This section treats of the reaction-intensities of the 

 hybrid as regards sameness, intermediateness, excess, and 



Table A 43. 



( Moral hydrate: 



M. voxillariu. . 



M. rcezlii 



M. bleuana. . . 

 Chromic acid: 



M. vexillaria. . 



M. ra>zlii 



M. bleuana. . . 

 Pyrogallic acid : 



M. vexillaria. 



M. rcezlii 



M. bleuana. . 

 Nitric acid: 



M. vexillariji ii 



Mra (V;; ..;;; 



, lie 



p /"oleuana 



CTilphuric acid: 



M. vexillaria 



M. rcezlii 



M. bleuana 



Hydrochloric acid: 



M. vexillaria 



M. rcezlii 



M . bleuana 



Potassium hydroxide: 

 M. vexillaria 



M. rcezlii 



M. blouana 



Potassium iodide: 



M. vexillaria 



M. rcezlii 



M. bleuana 



Potassium sulphocyanate: 



M. vexillaria 



M. rcezlii 



M. bleuana 



Potassium sulphide: 



M. vexillaria 



M. rcezlii 



M. bleuana 



Sodium hydroxide: 



M. vexillaria 



M. rcezlii 



M. bleuana 



Sodium sulphide: 



M. vexillaria 



M. rcezlii 



M. bleuana 



Sodium salicylate: 



M. vexillaria 



M. rcezlii 



M. bleuana 



( allium nitrate: 



M. vexillaria 



M. rcezlii 



M. bleuana 



Uranium nitrate: 



M. vexillaria 



M. rcezlii 



M. bleuana 



Strontium nitrate: 



M. vexillaria 



M. rcezlii 



M. bleuana 



( lobalt nitrate: 



M. vexillaria 



M. rcezlii 



M. bleuana 



Copper nitrate: 



M. vexillaria 



M. rcezlii 



M. bleuana 



Cupric chloride: 



M. vexillaria 



M. rcezlii 



M. bleuana 



Barium chloride: 



M. vexillaria 



M. rcezlii 



M. bleuana 



Mercuric chloride: 



M. vexillaria 



M. rcezlii 



M. bleuana 



98 



'... . 



67 



60 



62 



42 

 37 

 63 



87 97 99 



71 96 



1)7 



a > 



ao 95 



LOO 



99 



99 



100 

 100 



84 

 75 



'.(.' 



99 

 Ml 

 99 



95 



95 



83 



72 . . 

 96 



95 



87 

 98 



79 



58 



'J.'. 



so 



78 

 86 



M 



82 

 97 



:-:; 

 77 

 95 



91 



M, 



99 



16 



18 

 67 



M 

 73 



'.is 



56 

 52 



81 



2 



6 



10 



43 

 42 



7;. 



95 



99 



84! 

 97. 



99 



'..I 

 SO 

 99 



95 



98 



90 92 95 



85 s7 B9 

 99 



95 



95 96 



Ml '.Ml 

 99 



'-Mi 



95 96 

 83 I'll 

 99 



96 



10 12 

 18 22 

 30 i 33 



75 80 85 

 57 60 60 



97 98 I 98 



