606 



STARCHES OF LILIACEiE. 



Differentiation of Certain Starches of the Genus Brodicea. — Continued. 



PoijiRiscopic Pbopektiks. — Continued. 

 Figure. — Continued. 



B. capUala: Eccentric, very distinct, not always clear-cut, 



usually regular. 

 B. congesla: Eccentric, usually distinct, usually clear-cut, 



usually regular. 



Degree of Polarization. 



B. peduncularis: High. 



B. ixoides var. splendem: High, but slightly lower than in 



JS. peduncularis. 

 B. Candida: Fairly high, lower than in B. peduncularis. 

 B. lactea: High, slightly higher than in B. peduncularis. 

 B. laxa: High, not so high as in £. peduncularis. 

 B. coccinea: High, not so high as in B. peduncularis. 

 B. grandifiora: Fairly high, lower than in B. peduncularis. 

 B. califomica: Fairly high, lower than in B. peduncularis. 

 B. pwrdyi: High, about the same as in £. peduncularis. 

 B. stellaris: High, less than in B. peduncularis. 

 B. capilata: High, sUghtly higher than in B. peduncularis. 

 B. congesta: Fairly high, not so high as in B. peduncularis. 



P<^rization wUh Selenite — Quadrants and Colors. 



B. peduncularis: Quadrants not well defined, irregular in 

 shajje, unequal in size. Colors pure. 



B. ixoides var. splendens: Quadrants fairly well defined, 

 usually irregular in shape, imequal in size. Colors 

 usually not pure. 



B. ecmdida: Quadrants fairly well defined, irregular in 

 shape, unequal in size. Colors not quite pure. 



B. lactea: Quadrants well defined, sometimes irregular in 

 shape, unequal in size. Colors pure. 



B.laxa: Quadrants usually well defined, irregular in shape, 

 unequal in size. Colors usually pure. 



B. coccinea: Quadrants fairly well defined, often irregular 

 in shape, unequal in size. Colors usually pure. 



B. grandifiora: Quadrants usually not well defined, irreg- 

 ular in shape, unequal in size. Colors not pure. 



B. califomica: Quadrants not well defined, irregular in 

 shape, unequal in size. Colors not pure. 



B. purdyi: Quadrants usually not well defined, generally 

 irregular in shape, unequal in size. Colors fairly 

 pure. 



B. stellaris: Quadrants usually well defined, irregular in 

 shape, unequal in size. Colors usually pure. 



B. capitata: Quadrants well defined, regular in shape, un- 

 equal in size. Colors pure. 



B. congesta: Quadrants well defined, regular or irregular 

 in shape, unequal in size. Colors fairly pure. 



Iodine Reactions. 



Intensity and Color. 



B. peduncularis: Deep; blue-violet. 



B. ixoides var. splendens: Deep, same as in fi. peduncur 

 laris; blue-violet. 



B. Candida: Fairly deep, less than in B. peduncularis; blue- 

 violet. 



B. lactea: Deep, deeper than in B. peduncularis; blue- 

 violet. 



B. laxa: Deep, deeper than in B. peduncularis; blue-violet. 



B. coccinea: Very deep, deeper than in B. peduncularis; 

 blue- violet. 



B. grandifiora: Very deep, deeper than in B. peduncularis; 

 blue- violet. 



B. califomica: Deep, same as in B. peduncularis; blue- 

 violet. 



B. purdui: Very deep, deeper than in B. peduncularis; 

 blue-violet. 



B. stellaris: Deep, same as in JS. peduncularis; blue-violet. 



B. capitata: Very deep, deeper than in B. peduncularis; 

 blue- violet. 



B. congesta: Deep, same as in iS. peduncularis; blue-violet. 



Staining Reactions. 

 With Gentian Violet. 



B. peduncularis: Light. 



B. ixoides vai. splendens: Light j same as in B.peduncMtans. 



B. Candida: Fau-, deeper than m B. peduncularis. 



B. lactea: Light, deeper than in B. peduncularis. 



B. laxa: Light, deeper than in B. peduncularis. 



B. coccinea: Fairly deep, deeper than in B. peduncularis. 



B. grandifiora: Light, slightly more than in B. peduncu- 

 laris. 



B. califomica: Fairly deep, deeper than in B. pedxmcu- 

 laris. 



B. purdyi: Fairly deep, much deeper than in B. pedun- 

 cularis. 



B. stellaris: Light, same as in B. peduTWularis. 



B. capitata: Fairly deep, much deeper than in B. peduncu- 

 laris. 



B. congesta: Fair, deeper than in B. peduncularis. 



With Safranin. 



B. peduncularis: Light, but deeper than with gentian 

 violet. 



JS. ixoides var. splendens: Light, same aa in B. peduncu- 

 laris. 



JS. Candida: Light, but deeper than in B. peduncularis. 



B. lactea: Light, same as in £. peduncularis. 



B. laxa: Light, same as in B. peduncularis. 



B. coccinea: Fairly deep, much deeper than in B. peduncu- 

 laris. 



B. grandifiora: Fairly deep, much deeper than in B. pe- 

 duncularis. 



B. califomica: Light, the same as in B. peduncularis. 



B. purdyi: Fairly deep, much deeper than in B. peduncu- 

 laris. 



B. stellaris: Light, slightly deeper than in B. peduncularis. 



JS. capilata: Fair, deeper than in B. peduncularis. 



B. congesta: Light, slightly deeper than in B. peduncularis. 



Temperature Reaction. 



B. peduncularis: 68 to 69° C, mean 68.5°. 



B. ixoides var. splendens: 65.5 to 62° C, mean 61.75°. 



B. Candida: 61 to 63° C, mean 62°. 



B. lactea: 64 to 65° C, mean 64.5°. 



B. laxa: 60 to 62° C, mean 61°. 



JS. coccinea: 69 to 71° C, mean 70°. 



B. grandifiora: 65 to 66.8° C, mean 65.9°. 



B. califomica: 67 to 68° C, mean 67.5°. 



B. purdyi: 66 to 68° C, mean 67°. 



B. stellaris: 67.2 to 69° C, mean 68.1°. 



B. capitata: 65 to 66° C, mean 65.5°. 



B. congesta: 68.5 to 70° C, mean 69.25°. 



Effects op Various Reagents. 

 Reaction with Chloral Hydrate-Iodine. 



B. peduncularis: Begins in one-third of grains in 4 minutes; 



complete in one-third in 30 minutes. 

 B. ixoides var. splendens: Begins in five-sixths of grains in 



5 minutesj complete in five-sixths in 25 minutes. 

 B. Candida: Begins in half of grains affected in 5 minutes; 



complete in all in 35 minutes. 

 B. lactea: Begins in all in 5 minutes; complete in all in 45 



minutes. 

 B. laxa: Begins in all in 3 minutes; complete in all in 35 



minutes. 

 B. coccinea: Begins in two-thirds in 5 minutes; complete 



in all but 2 or 3 in 90 minutes. 

 B. grandifiora: All the small grains, a few of the large 



(equaling about half the total number), in 5 



minutes; complete in nearly three-fourths in 



90 minutes. 

 B. califomica: Begins in one-third in 5 minutes; com- 

 plete in one-third in 60 minutes. 



