GENUS HTMENOCALLIS. 



649 



Chart No. 219. 



IGV T 



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PS CI PA CA 

 PCPS 



The lamellce are not always demonstrable, especially near the hilum, but are distinct in a greater 

 number of grains than in H. undulata. When observed near the hilum they are in the form of 

 circular or oval rings, but most of the lamellae follow closely the outiine of the grain. One quite 

 refractive lamella is often seen at varying distances from the hilum, and sometimes there is a band 

 of very fine, indistinct lamellae at the margin of the grain. On the large grains frequently 16, rarely 

 20 to 22, lamellae can be counted. The lamellae are more distinct, but of the same general character 

 as in H. undulata. 



The grains vary in size; the smaller are 4 by 3m; the larger, elongated ones are 36 by 38//, and 

 the broadened forms are 36 by 44/i in length and breadth. The common size is about 24 by 22/« 

 in length and breadth. 



Polariscopic Properties. — The figure is centric, eccentric, or of the bean type; more often centric 

 or nearly centric than in H. undulata; and while the lines are of similar character and arrangement 

 with those of that species they are more often straight. 



The degree of polarization is high, rather higher than 

 in H. undulata. The same variation is found among the 

 different grains, but the proportion in which the polariza- 

 tion is very high is greater than in H. undulata. There is 

 less variation in the same aspect of a given grain than in 

 H. undulata. 



With selenite the quadrants are well defined, and 

 many are regular in shape and equal in size. The number 

 of grains in which they are regular is greater than in H. 

 undulata. The colors are generally pm-e, but sometimes 

 have a greenish tinge, or the margin of the yellow quad- 

 rant may be orange in tint. The colors are more often 

 pure than in H. undulata. 



Iodine Reactions. — With 0.25 per cent Lugol's solu- 

 tion the grains color a rather deep blue-violet, which 

 deepens rapidly, the tint being a little deeper and bluer 

 than in H. undulata. With 0.125 per cent solution they 

 become a light blue-violet, about the same as in H. undu- 

 lata, which deepens rapidly. After heating in water until 

 all the grains are gelatinized and then adding iodine, the solution becomes of a deep indigo-blue color 

 and the grains of a light reddish violet to a dull, fairly deep blue, many with a reddish tint. The 

 grains are deeper, redder, and duller in tint than in H. undulata. If the gelatinized grains are boiled 

 for 2 minutes and then treated with iodine, the solution colors very deeply and the grain-residues 

 a light to fairly deep blue, some with reddish tint, lighter than those of H. undulata, and the cap- 

 sules a light reddish violet. With an excess of iodine the grain-residues color a dull, fairly deep 

 blue with a reddish tint and the capsules a deep old-rose to a wine-red. The tint is slightly lighter 

 but rather redder than in H. undulata. 



Staining Reactions. — With gentian violet the grains begin to color slightly at once and in 30 

 minutes are lightly stained — shghtly lighter than in H. undulata. 



With safranin the grains begin to color at once and in 30 minutes are lightly colored — slightly 

 lighter than in H. undulata. 



Temperature Reaction. — The temperature of gelatinization is 68° to 69° C, mean 68.5°. 



Effects of Various Reagents. — With chloral hydrate-iodine reaction begins immediately. A few 

 grains are gelatinized in 30 seconds, about two-fifths in a minute, about four-fifths in 2 minutes, nearly 

 all in 3 minutes, and all but rare resistant grains in 5 minutes. In the latter the reaction is usually 

 complete in 14 minutes, rarely not until 17 minutes; it is qualitatively the same as in H. undulata. 



Reaction with chromic add begins immediately. A few grains are dissolved in 30 seconds, 

 more than half in a minute, nearly all in IJ/^ minutes, all but rare resistant grains in 2 minutes, and 

 all in 3 minutes. The reaction is qualitatively the same as in H. undulata. 



The reaction with pyrogallic acid begins immediately. A few grains are gelatinized in 15 sec- 

 onds, more than half in 30 seconds, nearly all in a minute, and all but rare resistant grains in IJ^ 

 minutes. In the latter the reaction is usually complete in 2 minutes, rarely 2J4 minutes; it is quali- 

 tatively the same as in H. undulata. 



Curve of Reaction-Intensities of Starch of 

 Hymenocallis calathina. 



