QBNU8 CR0CTT8. 



749 



Differentiation of Certain Starches of the Genua Crocus. — Continued. 



HiBTOLOOiCAL Characteristics. — Continued. 

 Conspicuous Forms. — Continued. 

 C. var {Baron von Brunow): Essentially the same as in 

 C. sxisianus, but the proportion of dome-shaped 

 and curvilinear grains is greater, the surface more 

 regular, and more doublets and triplets and quad- 

 ruplets of components of equal size. 



Hilum — Form, Number, and Position. 



C. ausianus {Clolh-of-Gold): Form round or lenticular; fre- 

 quently a short transverse or oblique cleft at hiium. 

 In dome-shai)ed grains frequently a longitudinal 

 fissure from sides of small cavity; rounded forms 

 may be a cross or a longitudinal fissure. Occa- 

 sionalljr Y-shaped or thorn-like. Position slightly 

 eccentric or centric; may be eccentric to 0.33 of 

 longitudinal axis. 



C. versicolor {Clolh-of Silver): The same as in C. stisianus 

 (Cloth-of-GoId), except the hilum is more often 

 demonstrable, and the fissures or cavities are 

 deeper. Position the same as in C. susianus (Cloth- 

 of-Gold). 



C. var. (Baron von Brunow): Essentially the same as in 

 C. versicolor (Cloth-of-Silver). Position the same 

 as in C. susianus (Cloth-of-Gold). 



Lamella — General Characteristics and Number. 



C. susianus {Cloth-of-Gold): Not demonstrable. 



C. versicolor (Cloth-of-Silver): Not usually demonstrable; 

 usually rings around hilum, tend to follow outline 

 of grain. 8 to 10 on grains of fair size. 



C. var. (Baron von Brunow): Not usually demonstrable. 



Size. 

 C. susianus (Cloth-of-Gold): From 3 to 22;i, commonly 12/*. 

 C. versicolor (Cloth-of-Silver): 3 to 24m, commonly 16m. 

 C. var. (Baron von Brunow): 3 to 18m, commonly 13m. 



PoLARiscopic Properties. 



Figure. 



C. susianus (Clolh-of-Gold): Usually slightly eccentric, 



lines usually not distinct through the entire figure 



and frequently bent or bisected. Sometimes 2 or 



more figures. 

 C. versicolor (Cloth-of-Silver): Same as in C susianiLs 



(Cloth-of-Gold), but more often regular and 



distinct throughout. 

 C. var. (Baron von Brunow): Same as in C. versicolor 



(Cloth-of-SUver). 



Degree of Polarization. 



C. susiamis (Cloth-of-Gold): Fair to high. 



C. versicolor (Cloth-of-Silver): Fair to very high, higher on 

 the whole than in C. susianus (Cloth-of-Gold). 



C. var. (Baron von Brunow): Fair to high, higher on the 

 whole than in C. susianus (Cloth-of-Gold), but less 

 than in C. versicolor (Cloth-of-Silver). 



Polarization with Selenite — Quadrants and Colors. 



C. susianus (Cloth-of-Gold): Quadrants usually not well- 

 defined, irregular in shape, and unequal in size. 

 Colors usually impure. 



C. versicolor (Cloth-of-Silver): Quadrants the same as in C. 

 susianus (Cloth-of-Gold), except that the quad- 

 rants are clear-cut, regular, and equal in a greater 

 portion of the grains. Colors usually impure. 



C. var. (Baron von Brunow): Quadrants the same as in C. 

 versicolor (Cloth-of-Silver) . Colors usually impure. 



Iodine Reactions. 

 Intensity and Color. 

 C. susianus (Cloth-of-Gold): Fairly deep; blue-violet. 

 C. versicolor (Cloth-of-SUver): Fairly deep, but deeper 

 than in C. susianus (Cloth-of-Gold); violet with 

 reddish tint. 



Iodine Reaotionb. — Continued. 



Intensity and Color. — Continued. 

 C. var. (Baron von Brururw): Fairly deep to Ught, less 

 deeply on the whole than in C. susianus (Cloth- 

 of-Gold); violet deep to light, redder than in C. 

 susianus (Cloth-of-Gold). 



Staininq Reactions. 

 With Gentian VioUl. 

 C. susianus (Cloth-of-Gold): Light. 

 C. versicolor (Clolh-of -Silver): Light, about the same as in 



C. susianus (Cloth-of-Gold). 



C. var. (Baron von Brunow): Light to fair, on the whole 



deeper than in C. susianus (Cloth-of-Gold). 



With Safranin. 



C. susianus (Clolhrof-Gold): Light to fair. 



C. versicolor (Cloth-of-Silver): Light to fair, about the 



same as in C. susianus (Cloth-of-Gold). 

 C. var. (Baron von Brurww): Light to fair, on the whole 

 deeper than in C. susianus (Cloth-of-Gold). 



Temperature op Gelatinization. 

 C. susianus (Clolh-of-Gold): 69 to 71° C^ mean 70°. 

 C. versicolor (Cloth-of-SUver): 75 to 76° C., mean 75°. 

 C. var. (Baron von Brunow): 71 to 73° C, mean 74". 



Effects of Various Reagents. 

 Reaction with Chloral Hydrate-Iodine. 



C. susianus (Cloth-of-Gold): Begins immediately; com- 

 plete in practically all in 3 minutes, and in all in 

 5}4 minutes. 



C. versicolor (Cloih-of Silver): Begins immediately; com- 

 plete in practically all in 7 minutes, and in all in 

 17 minutes. 



C. var. (Baron von Brunow): Begins immediately; com- 

 plete in practically all in 4 minutes, and in all in 

 11 minutes. 



. Reaction with Chromic Add. 



C. susianus (Cloth-of-Gold): Begins immediately; com- 

 plete in practically all in 45 seconds, and in all in 

 75 seconds. 



C. versicolor (Cloth-of-SUver): Begins immediately; com- 

 plete in practically all in 2^ minutes, and in all 

 m 4 minutes. 



C. var. (Baron von Brunow): Begins immediately; com- 

 plete in practically aJl in 1}4 minutes, and in all 

 in 2J^ mmutes. 



Reaction wUh Pyrogallic Acid. 



C. susianus (Cloth-of-Gold): Begins immediately; com- 

 plete in 30 seconds. 



C. versicolor (Cloth-of-SUver): Begins immediately; com- 

 plete in all in IJ^ minutes. 



C. var. (Baron von Brunow): Begins immediately; com- 

 plete in 50 seconds. 



Reaction with Ferric Chloride. 



C. susianus (Cloth-of-Gold): Begins in a few in 30 

 seconds; complete in practically all in 10 minutes 

 and in all in 25 minutes. 



C. versicolor (Cloth-of-SUver): Begins in a few in 60 

 seconds; complete in nearly all in 15 minutes, and 

 in all in 45 minutes. 



C. var. (Baron von Brunow): Begins immediately; com- 

 plete in practically all in 15 minutes, and in all 

 in IS minutes. 



Reaction wUh Purdy's Solution. 



C. susianus (Cloth-of-Gold): Begins immediately; com- 

 plete in practically all in 30 minutes. 



C. versicolor (Cloth-of-SUver): Begins immediately; com- 

 plete in about one-fourth in 30 minutes. 



C. var. (Baron von Brunow): Begins immediately; com- 

 plete in about two-tiurds in 30 minutei. 



