GENUS ANEMONE. 



867 



aart No. 372. 



With selenite the quadrants are clear-cut, fairly regular in shape, and usually unequal m size. 

 The colors are not pure. 



Iodine Reactions. — With 0.25 per cent Lugol's solution the grains color very deeply a blue-violet, 

 and with 0.125 per cent solution they color readily, and the color deepens rapidly. The reaction is 

 slightly less than that of A . apennina. After heating in water until the grains are all completely gelat- 

 inized, the solution colors faintly and the grains very deeply on the addition of iodine. After boiling for 

 2 minutes the solution colors deeply and the grains fairly. 

 The capsules color a red-violet with a very slight excess 

 of iodine and all of them contain blue-reacting starch. 



Staining Reactions. — With gentian violet the grains 

 begin to stain at once and in 30 minutes are fairly stained, 

 slightly deeper than those of A. apennina. 



With safranin the grains begin to stain at once and 

 in 30 minutes are lightly stained, more than those of 

 A. apennina. 



Temperature Reaction. — The temperature of gelatini- 

 zation is 62° to 64° C, mean 63°. 



Effects of Various Reagents. — With chloral hydrate- 

 iodine reaction begins at once and is over in IJ^ min- 

 utes. It is the same qualitatively as that of the grains 

 of A. apennina. 



The reaction with chromic acid begins at once and 

 is over in 15 seconds. It is the same qualitatively as 

 that of the grains of A. apennina. 



Reaction with pyrogallic acid begins at once and is 

 over in nearly all the grains in 3 minutes, while the rest 

 are almost completely gelatinized. It is the same qualitatively as that of the grains of A. apennina. 



With ferric chloride reaction begins in most grains in 30 seconds and is over 'va.2]/2 minutes. 

 It is the same qualitatively as that of the grains of A. apennina,. 



The reaction with Purdy's solution begins at once and is over in \l^ minutes. It is the same 

 qualitatively as that of the grains of A. apennina. 



Differentiation of Certain Starches of the Genus Anemone. 



HisTOLoaicAi, Characteristics. 

 Conspicuous Forms. 



A. apennina: Simple, no aggregates, no pressure facets, 

 surface 80inewh,at irregular owing to additions. 

 Elongated ovoid, elliptical, elliptical with one side 

 flattened, triangular with relatively very broad 

 base. 



A.fidgens: Essentially the same as in A. apennina, ex- 

 cept few well-defined pressure facets, some dome- 

 shaped. 



A. blanda: Essentially the same as in A. apennina, except 

 rare pressure facets. 



A. japonica: Simple, no aggregates, many have 1 or 2 or 

 more pressure facets, surface somewhat irregular. 

 Conspicuous forms dome-shaped to hemispherical 

 with 1 or usually 2 facets at the distal end. 



Hilum — Form, Number, and Position. 



A. apennina: Form fairly distinct, large, round, or len- 

 ticular; fissured, fissures small. Elongated hilum 

 appears to be double or triple. Position eccentric 

 0.33 of the longitudinal axis, or centric. 



A.fulgens: Form distinct, large, round or elongated, 

 usually single, may be multiple; usually fissured, 

 fissures simple or irregularly stellate. Position 

 eccentric about 0.33 of the longitudinal axis or 

 centric. 



A. blanda: Form not very distinct, round or elongated, 

 single or multiple, not fissured. Position eccentric 

 about 0.33 of the longitudinal axis, or centric. 



Histological Chabactebistics. — Continued. 

 Hilum — Form, Number, and Position. — Continued. 

 A. japonica: Form distinct, large round or triangular or 

 elongated, single; rarely fissured, fissures narrow 

 and shallow and not distinct. Position eccentric 

 about 0.40 of the longitudinal axis, or centric. 



Lamellce — General Characteristics and Number. 

 A. apennina: Invisible. 

 A.fulgens: Invisible. 

 A. blanda: Invisible. 

 A. japonica: Invisible. 



Size. 

 A. apennina: 2 to 14^1, commonly 10/j. 

 A.fulgens: 4 to 14m, commonly 10^. 

 A. blanda: 2 to 16m, commonly 10m. 

 A. japonica: 1 to 12m, commonly 8m. 



PoLARiscopic Properties. 

 Figure. 

 A. apennina: Centric or eccentric, not always distinct, 

 lines usually straight, clear-cut, usually irregular. 

 A. fulgens: The same as in .4. apennina. 

 A. blanda: The same as in .4. apennina. 

 A. japonica: The same as in .4. apennina. 



Degree of Polarization. 

 A. apennina: Low in most of the grains. 

 A. fulgens: Low in most of the grains, but higher than in 

 A. apennina. 



