PRACTICE XII (CONTINUED) 



1 In accurate work this pot should be covered with a layer of cotton, heated in an autoclave two hours 

 at 125, and planted with sterilized seed, assisting the young plants to push up through the cotton. 



2 Obtain about 0.5 kg. of soil which has recently grown the infected legume, and shake it up with about 

 1 liter of water. Let settle, and with each seed as it is planted add 10 cc. of the supernatant liquid before 

 the seed is covered. 



Why is the CaCOs added? Make observations and at least weekly notes of any differences in growth, 

 and explain. 



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