No. 4.] CATTLE COMMISSIONERS. 483 



in Brighton by Mr. Dennen and sent to an empty barn at 

 Bussey College, kindly placed at the disposal of the com- 

 mission by the courtesy of Dr. Theobald Smith. The bac- 

 teriological work has been done in Dr. Smith's laboratory 

 b} r him, and the Board is greatly indebted to him for his 

 advice and assistance. Much of the clinical work has been 

 done by Dr. A. W. May of Jamaica Plain, who has been of 

 great assistance in aiding in this portion of the experiments. 

 The following circular, sent out by the Bureau of Animal 

 Industry, describes the modus operandi : — 



United States Department of Agriculture, 

 Bureau of Animal Industry, Washington, D. C, April 21, 1900. 



The accompanying paper, prepared by Victor A. Norgaard, chief 



of the Pathological Division, entitled "Directions for the use of 



blackleg vaccine," is respectfully recommended for publication as 



Circular No. 23 (second revision) of this Bureau. Information 



of this character first appeared as Circular No. 21. 



Very respectfully, D. E. Salmon, 



Chief of Bureau. 



Approved : James Wilson, 



Secretary of Agriculture. 



Directions for the Use of Blackleg Vaccine. 



[By VICTOR A. Norgaard, Chief of Pathological Division, Bureau of Animal 



Industry.] 



The blackleg vaccine, as prepared by this Bureau, consists of a 

 brownish powder, which is put up in packets containing either ten 

 or twenty-five doses each. To prepare this powder in such a way 

 that it may be injected hypodermically, it is necessary to obtain 

 certain implements, which, together with the hypodermic syringe, 

 are known as a vaccinating outfit. This consists of a porcelain 

 mortar with pestle, a small glass funnel and a measuring glass. 

 For filtering the vaccine we have found absorbent cotton to be 

 most suitable. Fig. 1 is an illustration of the vaccinating outfit 

 recommended by this Bureau. All of the utensils, including the 

 hypodermic syringe and a package of absorbent cotton, are fitted 

 in a strong polished oak box, which, by means of an adjustable 

 wire loop, serves also as a support for the funnel when the vaccine 

 is filtering. The syringe, two hypodermic needles and an extra 

 glass barrel are packed in a separate metal box, which is inclosed 

 in the oak box. 



