No. 4.] CATTLE COMMISSIONERS. 491 



(5) Place cotton in glass funnel, and moisten with water. 



(6) Filter vaccine into the glass or bottle. 



(7) Secure the animal to be injected. 



(8) Iusert the needle through the skin. 



(9) Fill the syringe, and adjust the screw regulator on the 

 piston. If the first animal is a yearling or older, place regulator 

 No. 1 on the syringe (fig. 2) . 



(10) Fit the peg of the syringe into the cap of the needle, and 

 inject the dose. 



(11) Withdraw syringe and needle together. If the syringe is 

 removed from the needle before this has been drawn out of the 

 skin, some of the injected vaccine will flow back through the 

 needle, and be lost. In this case the animal does not get its full 

 dose, and will consequently be insufficiently protected. 



Attention. 



(1) Cattle owners are hereby requested to report immediately all 

 deaths from blackleg which occur ivithin one week after vaccination. 

 For this purpose a separate blank and return envelope (which 

 requires no postage) are furnished with the vaccine. 



(2) Vaccine ivhich for any reason is not used should be returned 

 to this office immediately, and in acknowledgment of the same a 

 new application blank will be forwarded to the sender. 



(3) Do not castrate or dehorn at the time of vaccination. 



The vaccine material is made by the following method, at 

 the Lyon's Veterinary School : " Forty grammes of infected 

 muscle are rapidly dried at a temperature of 32° C, and 

 are intimately mixed with eighty grammes of water. This 

 mixture is divided into twelve equal parts, which are placed 

 on separate flat plates. These plates are put into a ther- 

 mostat for six hours to dry ; six plates are exposed to a 

 temperature of 100° C, in order to produce the weaker 

 or first material, and the remaining plates to one of 85° C, 

 to obtain the stronger or second material. The inoculation 

 is made in two stages, first with the weaker and afterward 

 with the stronger material. The dried-up brown crust on 

 the plates is used for the inoculation, and may be kept for 

 a moderately long period." (See Hayes' "Translation of 

 Friedberger and Frohner," Vol. I., page 128.) 



Most of the vaccine viruses in use are prepared in this 



