II. METHODS. 



My presorved material was collected and prepared for 

 study as follows:- By hanging boxes and otlier structures 

 of wood in the water at Beaufort , I was able to obtain all 

 stages from the newly-attachod larva to ". .ip-v;orms" four 

 inches long, v/ith adult organization. The yoiongest stages 

 were collected from the surface of the vrood. Later stages 

 were dissected ov^t of the wood into wtiich they had bored. 

 Early stages were narcotized with cocain and aftervmrds 

 fixed v;ith mercuric chloride. Specimens 1 cm. or more in 

 length v;ere treated as follows: They- vrere exposed quick- 

 ly and immediately a quantity of Hermann's solution was 

 dashed upon them. This kills them instantly, before they 

 have had time to contract appreciably. They were then im- 

 mediately immersed in mecuric chloride or Perenyis' solu- 

 tion for fixation. After '.vashing in weaker alcohols, spe- 

 cimens were preserved in 90^ alcohol. The early stages 

 were stained in Kleinenberg's hematoxylin. For later 

 stages, the best results were obtained with borax-carmine, 

 follov/ed by Lyons blue. For the examination of whole ob- 

 jects, the best results were obtained by stainin;: in a 

 weak solution of borax- cannine in acid {1-^"'''' CI. )70', al- 



