IIMM).] PUBLIC DOCUMENT— Xo. ;U. l*;") 



235.66 mgs. KoH required to saponify 1 gram fsit. 

 213.89 mg.s. KoII equals saponification number of insoluble fats. 

 86.()() equals per cent, of insoluble acids. 



.8600X213.89= 185.35 mgs. KoH to saponify insoluble acids 

 in 1 gram fat. 

 235.66 — 185.35= 50.30 mgs. Koll refjuired to saponify soluble 

 acids in 1 gram fat. 

 50.30 -f- .0803 = 626.46 theoretical saponification number of 

 soluble acids. 



The mean molecular weight of the soluble acids was calculated 

 by the same formula as in case of the insoluble acids. 



The mean molecular weight of the volatile acids was de- 

 termined by evajjorating to dryness the titrated portion resulting 

 from the lieichert-Mcissl number. Erom the weight of the 

 salts and of the alkali present in them the mean molecular 

 weight can be readily calculated by the following formula : — 



_ 40.058 [.salts— (e.c. N/lONaOH X .0040058)] 



c.c. N/10 NaOH X .0040058 '^ 18.01b 



10000 [salts— (c.c. N/10 NaOH X .0040058)] 



C.C. N/10 xNaOH. +18.016 



Every precaution was talven and blanli determinations were 

 made on all of the reagents and deducted. 



The sa[)Ouification number (S) of the volatile fatty acids 

 was obtained from the mean molecular weight (M), as fol- 

 lows : — 



c 56158 



The figures which follow represent the averages of analyses 

 made in the experiment described. Number 1, includes an 

 average of all the data exeei)ting those from Herd II. in the 

 third or soy bean oil period. Because the soy bean oil so dis- 

 tinctly modified the composition of the fat the average for this 

 period is presented by itself under jSTumber II. Number III. 

 represents the analysis of a sample of superior butter sent by 

 Gude Brothers of Xew York. Number IV. represents the 

 maximum and minimum tests usually recognized, with the 

 approximate averages. 



