HIPPEASTRUM. 



407 



observed than in //. cleonia. A delicate fissure proceeds 

 from the hilum previous to the beginning of gelatiniza- 

 tion in some grains; as in II. titan, but more frequently 

 than in //. cleonia ; and irregular fissures sometimes form 

 throughout the main body of the grain, which were not 

 noted in the parents. 



The gelatinized grains are swollen and distorted so 

 that they do not resemble the form of the untreated grain 

 as in the parents. 



In the reaction with sodium salicylate II. titan-cleonia 

 shows qualitatively a very close relationship to both 

 parents, but is somewhat closer to II. titan than to //. 

 cleonia. Any characteristic which appears in one or both 

 parents is often further developed in the hybrid. 



The reaction with calcium nitrate begins in rare 

 grains in 1 minute. Complete gelatinization occurs in 

 less than 0.5 per cent of the entire number of grains and 

 total starch in 5 minutes; still in less than 0.5 per cent 

 ol the grains and total starch in 15 minutes; in about 

 1 per cent of the grains and 2 per cent of the total starch 

 in 30 minutes ; little if any further advance in 45 and 60 

 minutes, respectively. (Chart D 35.) 



The reaction with uranium nitrate begins in rare 

 grains in 1 minute. Complete gelatinization occurs in 

 about 0.5 per cent of the grains and 1 per cent of the 

 total starch in 5 minutes; very slight progress in 15 and 

 30 minutes ; in about 1 per cent of the grains and 2 per 

 cent of the total starch in 45 minutes ; little if any further 

 advance in 60 minutes. (Chart D 36.) 



The reaction with strontium nitrate begins in rare 

 grains in 1 minute. Complete gelatinization occurs in 

 less than 0.5 per cent of the entire number of grains and 

 total starch in 5 minutes; in about 0.5 per cent of the 

 grains and 1 per cent of the total starch in 15 minutes; 

 in about 1 per cent of the grains and 3 per cent of the 

 total starch in 30 minutes; in about 2 per cent of the 

 grains and 5 per cent of the total starch in 45 minutes ; in 

 about 3 per cent of the grains and 7 per cent of the total 

 starch in 60 minutes. (Chart D 37.) 



The reaction with cobalt nitrate begins in very rare 

 grains in 1 minute. Complete gelatinization occurs in 

 less than 0.5 per cent of the grains and total starch in 5 

 minutes; still less than 0.5 per cent of the grains and 

 1 per cent of the total starch in 15 minutes; in about 0.5 

 per cent of the grains and total starch in 30 minutes; 

 little if any further progress in 45 and 60 minutes, 

 respectively. (Chart D 38.) 



The reaction with copper nitrate begins in rare grains 

 in 1 minute. Complete gelatinization occurs in less than 

 0.5 per cent of the entire number of grains and total 

 starch in 5 minutes; in about 1 per cent of the grains 

 and 2 per cent of the total starch in 15 minutes; little if 

 any further progress in 30, 45, and 60 minutes, respec- 

 tively. (Chart D 39.) 



The reaction with cupric chloride begins in very rare 

 grains in 1 minute. Complete gelatinization occurs in 

 less than 0.5 per cent of the entire number of grains and 

 total starch in 5 minutes; very slight progress in 15 min- 

 utes; and in about 0.5 per cent of the entire number 

 of grains and 1 per cent of the total starch in 30 minutes ; 

 in about 1 per cent of the grains and 2 per cent of the 

 total starch in 45 minutes ; little if any further progress 

 in 60 minutes. (Chart D 40.) 



The reaction with barium chloride begins in very rare 

 grains in 1 minute. Complete gelatinization occurs in 

 less than 0.5 per cent of the entire number of grains and 

 total starch in 5 minutes; very slight progress in 15, 30, 

 45, and 60 minutes, until at the end of which period still 

 less than 0.5 per cent of the entire number of grains 

 and total starch is gelatinized. (Chart D41.) 



The reaction with mercuric chloride begins in rare 

 grains in 1 minute. Complete gelatinization occurs in 

 less than 0.5 per cent ol' the entire number of grains 

 and total starch in 5 minutes; slight progress in 15 and 

 30 minutes; in about 1 per cent of the entire number 

 of grains and 2 per cent of the total starch in 45 minutes ; 

 little if any further progress in 60 minutes. (Chart D42.) 



3, Staeciies of Hippeastktjm ossultan, H. pyrriia, 



AND H. OSSULTAN-PYRRHA. 



HlPPEASTRUM OSSULTAN (SEED PARENT). 

 (Plate 2, fig. 10; Charts D 43 to D 63.) 

 Histologic Properties. 

 In form the grains are usually simple and isolated, 

 but there is a moderate number of compound grains 

 which may consist of from 2 to 8 or more components, 

 and also a few aggregates are noted consisting of 2 or 3 or 

 more grains linearly arranged. The grains are moder- 

 ately regular in form, but a perfect regularly formed 

 grain is not common. The irregularities noted are due 

 to the following causes: (1) A set of secondary lamellae 

 whose longitudinal axis is at an angle to that of the 

 primary grain; (2) shallow depressions in and flattening 

 of the margin; (3) small nipple-like protuberances; 



(4) 1, 2, or 3 pressure facets on the distal end and sides ; 



(5) very irregular forms, due to an irregular mass of 

 small grain forming a large compound grain. The con- 

 spicuous forms are : Bound and nearly round, ovoid with 

 one pointed end or with both ends blunt, and elliptical. 

 There are also lenticular, irregularly quadrilateral, trian- 

 gular, plano-convex, and modified reniform shapes. 



The hilum is a round or lenticular spot and is moder- 

 ately distinct and not often fissured. When the fissure 

 occurs it takes the following forms : A small, indistinct, 

 straight, angled, or curved line. The hilum is sometimes 

 centric but is commonly eccentric 0.45 to 0.3, usually 

 0.38, of the longitudinal axis. 



The lamella are usually not very distinct, but on 

 many grains they are distinct and appear as rather coarse, 

 continuous bands, all of which have the form of the 

 outline of the grain, though this may be somewhat modi- 

 fied in individual cases. There are often grains in which a 

 secondary deposit of starch has occurred, and the lamella? 

 of this secondary deposit may or may not be continuous, 

 but are usually coarser and more distinct than those of 

 the primary formation. The number counted on the 

 common-sized and larger grains varies from 7 to 12, 

 usually 10. 



The grains vary in size from the smaller which are 4 

 by 4;u to the larger broader forms which are 44 by 38/u. 

 and the larger elongated forms which are 42 by 34ft and 

 rarely 44 by 24/x in length and breadth. The common 

 sizes are 26 by 26yu, and 30 by 24^. 



Polauiscopic Properties. 



The figure varies from centric to very eccentric, 

 with the majority slightly eccentric. The lines vary 



