NERINE. 



491 



Nerine Queen of Roses (Hybrid). 



(Plate 6, fig. 36; Charts D 190 to D 210.) 

 Histologic Properties. 



In form the grains are usually simple and isolated, 

 but there are more aggregates containing more component 

 grains, and more compound grains, than in either parent 

 or in N. dainty maid. The compound grains are of the 

 character described under N. elegans. The grains are 

 less regular than those of N. elegans, but more regular 

 than those of N. crispa and N. dainty maid. The causes 

 of any irregularities are the same as in N. elegans, ex- 

 cept that notches in the distal margin are not so common 

 as in that starch. The conspicuous forms are ovoid and 

 elliptical with flattened distal end, even among the small 

 grains, in which respect it resembles N. elegans; however, 

 there are more small, round grains than in that starch. 

 There are also pyriform, triangular with rounded angles, 

 and a few of the spatulate forms seen in N. crispa. 



The grains of this hybrid are more like N. elegans 

 than N. crispa in form. This hybrid differs from N. 

 dainty maid, in a greater resemblance to N. crispa in 

 being more regular in form, and in having more aggre- 

 gates and compound grains. 



The liilum is as distinct as in N. crispa and N. dainty 

 maid, but is very rarely fissured, and in the latter respect 

 is more like N. elegans and N. dainty maid. The hilum 

 is eccentric from 0.36 to 0.17, usually 0.21 of the longi- 

 tudinal axis, more than in either parent. The character 

 and the eccentricity of the hilum are, on the whole, more 

 like N. elegans than N. crispa. 



N. queen of roses and N. dainty maid more closely 

 resemble one another in the character of the hilum than 

 either resembles either parent, but both are nearer N. 

 elegans than N. crispa. 



The lamella; are about as fine and as distinct and are 

 of the same character and arrangement as in N. crispa; 

 the number counted on the larger grains varies from 16 

 to 30, usually 24; in character and arrangement they are 

 closer to N. crispa than to N. elegans, but in number 

 closer to N. elegans. The lamellae of N. queen of roses 

 are nearer, on the whole, to N. crispa than are those of 

 N. dainty maid, and the latter are nearer to N. elegans 

 than to N. crispa. 



The size of the grains varies from 3 by 3/* for the 

 smaller to 44 by 28/x in length and breadth for the larger. 

 The common size is 22 by 16/n. In size N. queen of roses 

 is smaller than either parent, but nearer N. elegans than 

 N. crispa. N. queen of roses is smaller than N. dainty 

 maid, but slightly closer to N. dainty maid than the 

 latter is to either parent, but not so close to N. elegans 

 as is N. dainty maid. 



Polariscopic Properties. 



The figure is slightly to very eccentric, usually eccen- 

 tric, distinct, and clear-cut as in iV. elegans. The lines 

 are commonly finer than in N. elegans but intersect 

 obliquely, and are bent and bisected as in N. elegans. 



The degree of polarization is moderate to very high 

 (value 77), less than in either parent or in N. dainty 

 maid, and there are more of the moderately high than in 

 either parent or in N. dainty maid. 



With selenite the quadrants are usually well defined, 

 and often somewhat more irregular in shape than in N. 



elegans and always unequal in size. The colors are less 

 often pure than in N. elegans, and the yellow is less often 

 pure than the blue. A very few of the grains have a 

 greenish tinge. 



In degree of polarization, character of the figure, and 

 appearances with selenite N. queen of roses is closer to 

 N. elegans than to N. crispa, and closer to N. elegans 

 in all polariscopic characteristics than in N. dainty maid. 



Iodine Reactions. 



With 0.25 Lugol's solution the grains color a mod- 

 erate blue with a tinge of violet (value 55), the same 

 as in N. elegans, but deeper than in N. crispa and not 

 so deep as in N. dainty maid, and the color rapidly 

 deepens to very deep as in N. elegans. With 0.125 per 

 cent Lugol's solution, the grains color a moderate to 

 light blue with a tinge of violet, the same as in N. elegans 

 and the color deepens, as in those grains, to a moderately 

 deep to deep. After heating in water until the grains 

 are gelatinized, and then adding 2 per cent Lugol's 

 solution, the grains color a moderately deep to very deep 

 indigo-blue, as in N. crispa; the capsules usually can not 

 be distinguished, but when they can they color a purer 

 violet as in 2V. crispa; the solution colors a moderately 

 deep indigo-blue as in N. crispa. If the preparation is 

 boiled for 2 minutes, and then treated with an excess of 

 2 per cent Lugol's solution, the grain-residues color light 

 to deep indigo-blue, as in N. crispa, the capsules (not all 

 of which can be distinguished) a pure violet as in N. 

 crispa, and the solution a deep indigo-blue. 



Quantitatively and qualitatively the iodine reactions 

 of the unheated grains of N. queen of roses are closer to 

 N. elegans, and qualitatively the reaction of the gelati- 

 nized grains arc closer to N. crispa. 



The iodine reactions of the unheated grains of JV. 

 queen of roses are closer to N. elegans than are those 

 of N. dainty maid; and those of the gelatinized grains 

 more closely resemble N. crispa, while those of N. dainty 

 maid resemble N. elegans. 



Aniline Reactions. 



With gentian violet the grains color very lightly at 

 once, and in half an hour they are light to moderately 

 colored (value 40), the same as in N. crispa. As in the 

 parents there is inequality of coloring between different 

 grains and in individual grains. 



With safranin the grains color very lightly at once, 

 and in half an hour they are moderately colored (value 

 50), the same as in N. crispa. As in the parents there 

 is inequality of coloring between different grains and in 

 individual grains. 



In the reactions to aniline stains, N. queen of roses 

 shows a closer relationship to N. crispa than to N. ele- 

 gans; it is closer to N. crispa than to N. dainty maid, 

 which in turn is closer to iV. elegans. 



Temperature Reactions. 



The majority of the grains are gelatinized at 68° 

 to 69.1° C, and all at 71° to 72.8° C, mean 71.9° C, 

 or 1.2° higher than in N. crispa, 1° lower than in N. 

 elegans, and 1.3° C. lower than in N. dainty maid. 



The temperature of gelatinization of N. queen of roses 

 is intermediate between the parents and closer to N. 

 crispa, than to N. elegans. and it is closer to N. crispa 

 than is that of N. dainty maid. 



