NERINE. 



505 



JV. giantess, but considerably more irregular than those of 

 JV. sarniensis var. corusca major, and the irregularities 

 are due to the same causes as described under the parents. 

 The conspicuous forms are narrow, elongated or broad 

 elliptical with or without a flattened distal end, ovoid, 

 clam-shell-shaped, triangular, and large irregularly quad- 

 rilateral ; and, among the smaller grains, round or nearly 

 round, elliptical, pyriform, and lenticular. These grains 

 appear to be very nearly mid-intermediate between those 

 of the parents, but they are slightly nearer those of JV. 

 sarniensis var. corusca major. They are in form closer 

 to those of JV. sarniensis var. corusca major, and not so 

 near those of JV. bowdeni as those of iV. giantess. The 

 grains of the two hybrids closely resemble one another. 



The hilum is as distinct as in JV. bowdeni, but less 

 frequently fissured than in those grains; when Assuring 

 occurs it is of the types noted under JV. sarniensis var. 

 corusca major. The hilum is sometimes centric, but is 

 usually eccentric from 0.44 to 0.2, commonly 0.25, of the 

 longitudinal axis, somewhat greater than in either parent. 

 The hilum in character is slightly nearer to JV. bowdeni, 

 and in eccentricity to N. sarniensis var. corusca major, 

 and nearer JV. bowdeni than is N. giantess. 



The lamella; are not so distinct or so irregular as in 

 N. bowdeni and JV. giantess, but more distinct and 

 slightly more irregular than in JV. sarniensis var. corusca 

 major. In arrangement they are more like those of the 

 grains of JV. sarniensis var. corusca major. The number 

 counted on the larger grains varies from 16 to 24, usually 

 18 — a notably less number than in either parent. In 

 character and arrangement of the lamella?, these grains 

 are nearer N. sarniensis var. corusca major than JV. 

 bowdeni, and the grains of this hybrid differ from those 

 of JV. giantess which are nearer JV. bowdeni, while these 

 are nearer JV. sarniensis var. corusca major. 



The grains in size vary from the smaller ones which 

 are 8 by 8/x and 10 by 8/*, to the larger elongated forms 

 which are 56 by 44/* and 56 by 34/t, and the larger broad 

 forms which are 42 by 52/i, in length and breadth. The 

 common sizes are 36 by 20,u, 34 by 32^, and 28 by 32/x. 

 These grains are, on the whole, intermediate in size be- 

 tween those of the parents, but closer to JV. bowdeni than 

 JV. sarniensis var. corusca major, and also closer than 

 are those of JV. giantess. 



Polariscopic Properties. 



The figure is nearly centric to very eccentric, more 

 eccentric than in either parent, and as distinct and more 

 clear-cut than in JV. bowdeni. The lines are as fine as in 

 JV. bowdeni, but finer than in JV. sarniensis var. corusca 

 major, and they intersect obliquely with less variation 

 of the angle than in JV. bowdeni, but more than in JV. 

 sarniensis var. corusca major. They are also less apt to 

 be bent or bisected than in either JV. bowdeni or JV. 

 giantess, but more than in JV. sarniensis var. corusca 

 major. 



The degree of polarization is moderately high to very 

 high (value 80), less than in either parent and the same 

 as in N. giantess. There is comparatively little variation 

 in polarization in the same aspect of a given grain as in 

 JV. sarniensis var. corusca major and JV. giantess. 



With selenite the quadrants as in JV. giantess are 

 usually well defined, unequal in size, and less irregular in 

 shape than in JV. bowdeni, but considerably more than in 

 9 



JV. sarniensis var. corusca major. The colors are not eo 

 pure as in JV. giantess, but somewhat more pure than in 

 JV. bowdeni. 



In the degree of polarization, the character of the 

 figure, and the appearances with selenite JV. abundance, 

 is closer to JV. bowdeni than to JV. sarniensis var. corusca 

 major. A', abundance is nut so near JV. bowdeni, as is 

 JV. giantess, but both hybrids are very close to one another. 



Iodine Reactions. 



With 0.25 per cent Lugol's solution, the grains color 

 a moderate blue-violet (value 50), the same as JV. bowdeni 

 in depth and in tint. With 0.125 per cent Lugol's solu- 

 tion, they color a light blue-violet, less than in JV. bow- 

 deni, which deepens rapidly, becoming more blue. After 

 heating in water, until all the grains are gelatinized and 

 then adding 2 per cent Lugol's solution, the grains color 

 a very light to deep indigo-blue, lighter than in JV. bow- 

 deni; the capsules, which can frequently be seen, color 

 a pure violet; and the solution a deep indigo-blue, more 

 than in JV. bowdeni. If the preparation is boiled for 2 

 minutes, and then treated with an excess of 2 per cent 

 Lugol's solution, the grain-residues color a very light to 

 deep indigo-blue with a tinge of red less than in JV. 

 bowdeni, the capsules a pinkish violet as in JV. bowdeni, 

 and the solution a very deep indigo as in JV. bowdeni. 

 Qualitatively and quantitatively the iodine reactions of 

 JV. abundance are closer to JV. bowdeni than to JV. sar- 

 niensis var. corusca major. 



Quantitatively the iodine reactions of the unheated 

 grains of JV. abundance and JV. giantess are as far apart 

 as are the parents, but both quantitatively and qualita- 

 tively the gelatinized grains of the two hybrids are very 

 close to one another and to JV. bowdeni. 



Aniline Reactions. 



With gentian violet the grains stain, lightly at once, 

 and in half an hour they are light to moderately stained 

 (value 40), the same as JV. sarniensis var. corusca major. 



With safra7iin the grains stain lightly at once, and 

 in half an hour they are moderately stained (value 45) 

 less than JV. bowdeni, but more than in JV. sarniensis var. 

 corusca major. 



The aniline reactions of this starch show a closer 

 relationship to JV. sarniensis var. corusca major. These 

 grains are less light than those of JV. giantess, and there- 

 fore nearer JV. sarniensis var. corusca major in this re- 

 spect, while those of JV. giantess are closer to JV. boivdeni. 

 They are closer to one another, however, than the parents 

 are to one another. 



Temperature Reactions. 



The majority of the grains are gelatinized at 69° 

 to 69.9° C, and all at 73.9° to 74.8° C, mean 74.3° C. 



The temperature of gelatinization of JV. abundance 

 is much closer to JV. bowdeni than to JV. sarniensis var. 

 corusca major, and is higher than that of JV. giantess and 

 is also nearer JV. bowdeni. 



Effects of Various Reagents. 

 The reaction with chloral hydrate begins immediately. 

 Complete gelatinization occurs in about 24 per cent of the 

 entire number of grains and 45 per cent of the total 

 starch in 5 minutes; in about 75 per cent of the grains 

 and 82 per cent of the total starch in 15 minutes; in 



