NARCISSUS. 



533 



Narcissus poetaz TUiuJirn (Hybrid). 



(Plate 10, fig. 57; Charts D 205 to D 280.) 

 Histologic Pbofebtees. 



In form the grains are usually simple and isolated. 

 Compound grains and aggregates are not so frequently 

 seen as in JV. tazella grand monarquc, but more fre- 

 quently than in JV. posticus ornatus. The compound 

 grains belong to two types, which are seen in both 

 parents: (1) 2 moderate-sized grains inclosed in 2 or 

 3 common secondary lamellae ; (2) a large gram, to the 

 side or distal end of which a small grain has become 

 adherent, and both later inclosed in 1 or 2 common 

 secondary lamellae. The aggregates have the same num- 

 bers and kinds of grains and arrangement as in JV. 

 tazetta grand monarquc. There are also somewhat fewer 

 simple grains than in JV. tazetta grand monarquc in 

 which a clear differentiation can be made between a 

 primary and a secondary starch formation; but there 

 are more than in JV. poeticus ornatus. The grains are 

 more irregular in form than in either parent, in which 

 respect they more nearly resemble JV. tazetta grand mon- 

 arquc, and the irregularities are due to the same causes 

 as in the parents. The conspicuous forms are ovoid, 

 triangular (usually scalene), plano-convex, pyriform, 

 and dome-shaped. The additional forms are reniform, 

 spindle-shaped, lenticular, quadrilateral with rounded 

 angles, and club-shaped. The grains are as often flat- 

 tened as in N. tazetta grand monarquc, and more often 

 than in JV. poeticus ornatus. In form JV. poetaz triumph 

 shows a somewhat closer relationship to JV. tazetta grand 

 monarquc than to JV. poeticus ornatus. 



The hilum when not fissured is as distinct as in JV. 

 poeticus ornatus. It is more often fissured and the 

 fissures are deeper and more extensive than in either 

 parent, and in this respect the resemblance is somewhat 

 closer to JV. poeticus ornatus. The fissures have the same 

 arrangement as in both parents. The hilum is sometimes 

 centric, but in the majority of the grains it is eccentric 

 from 0.42 to 0.25, usually 0.35, of the longitudinal axis. 

 In the character of the hilum JV. poetaz triumph shows 

 a somewhat closer relationship to JV. poeticus ornatus 

 than to JV. tazetta grand monarque. The degree of eccen- 

 tricity is the same in all three starches. 



The lamella; are as often not distinct, and are as 

 fine and have the same general arrangement as far as 

 could be observed as in JV. tazetta grand monarque. The 

 number on the grains can not be determined. In the 

 character of the lamella? JV. poetaz triumph shows a 

 somewhat closer relationship to JV. tazetta grand mon- 

 arque than to JV. poeticus ornatus; but there are no 

 great differences between either parents and hybrid with 

 regard to the lamellae. 



The grains in size vary from the smaller which are 

 4 by 4/i to the larger broad forms which are 34 by 42/i 

 and the larger elongated forms which are 40 by 28/a in 

 length and breadth. The common sizes are 24 by 30/j. 

 and 32 by 26/*. In size and proportions the grains show 

 a somewhat closer relationship to JV. tazetta grand mon- 

 arquc than to JV. poeticus ornatus, though the common 

 size is larger than in either parent. 



PoLARisconc Properties. 

 The figure is moderately distinct and as poorly de- 

 fined as in N. poeticus ornatus. The lines usually cross 



at an acute angle which is not very variable in size, and 

 they are sometimes bent and bisected as in N. poe 

 ornatus. The figure has sometimes the form of an hyper- 

 bola or a long line bisected at both ends. 



The degree of polarization varies from low to very 

 high (value 50), the same as in both parents. There 

 is the same amount of variation in the same aspect of a 

 given grain. 



With selenite the quadrants are as poorly defined and 

 as irregular in shape and as unequal in size as in N. 

 poeticus ornatus. The colors arc usually not pure as in 

 both parents. 



In the character of the figure and in the appearances 

 with selenite, N. poetaz triumph shows a somewhat closer 

 relationship to JV. poeticus ornatus than to JV. tazetta 

 grand monarque. There is no difference to be noted in 

 the degree of polarization of the three starches. 



Iodine Reactions. 

 With 0.25 per cent Lugol's solution, the grains all 

 color a light to moderate violet tinged witli blue (value 

 40), the same as in JV. poeticus ornatus, and then deepen 

 with moderate rapidity until all the grains are very deeply 

 colored and have assumed more of a bluish tint. With 

 0.125 per cent Lugol's solution, the grains all color a 

 light violet, the same as in JV. poeticus ornatus, the color 

 deepens with moderate rapidity until they are deeply 

 colored and have assumed a bluish tinge. After heating 

 in water until all the grains are completely gelatinized 

 and then adding a 2 per cent Lugol's solution the grains 

 all color a moderate or moderately deep indigo-blue, and 

 the solution colors a moderate indigo-blue as in JV. 

 poeticus ornatus. If the preparation is boiled for 2 

 minutes and then treated with an excess of 2 per cent 

 Lugol's solution, the grain-residues color a light to mod- 

 crate indigo-blue, the capsules a red or reddish violet, 

 and the solution a very deep indigo as in JV. poeticus 

 ornatus. Qualitatively and quantitatively the reaction of 

 JV. poetaz triumph with iodine shows a closer relationship 

 to JV. poeticus ornatus than with JV. tazetta grand 



monarque. 



Aniline Reactions. 



With gentian violet the grains all color very lightly 

 at once, and in 30 minutes they are light to moderately 

 colored (value 32), less than in either parent, but closer 

 to JV. poeticus ornatus. 



With safranin the grains all color very lightly at 

 once, and in 30 minutes they are light to moderately 

 colored (value 40), less than in either parent whose 

 values correspond. In the reaction with gentian violet 

 JV. poetaz triumph shows a closer relationship to JV. poeti- 

 cus ornatus than to JV. tazetta grand monarque. Noth- 

 ing is shown by the reaction with safranin. 



Temperature Reactions. 



The temperature of gelatinization of the majority 

 of the grains is 73° to 75° C, and of all is 76° to 77° C, 

 mean 76.5° C. 



The temperature of gelatinization of JV. poetaz 

 triumph is the same as that of JV. tazetta grand monarque. 



Effects of Various Reagents. 

 The reaction with chloral hydrate begins in 1 minute. 

 Complete gelatinization occurs in about 1 per cent of 

 the entire number of grains and 4 per cent of the total 



