NARCISSUS. 



541 



same as in N. gloria muudi, and the color deepens with 

 moderate rapidity until the grains are very deeply 

 colored with a much bluer tint. With 0.125 per cent 

 LugoFs solution, the grains all color a light violet, the 

 same as in N. gloria mundi, and the color deepens with 

 moderate rapidity until they are deeply colored. If the 

 grains are heated in water until they are completely 

 gelatinized and then treated with a 2 per cent Lugol's 

 solution the gelatinized grains color a moderate or a 

 moderately deep indigo-blue and the solution a deep 

 indigo-blue as in both parents. If the preparation is 

 boiled for 2 minutes and then treated with an excess 

 of 2 per cent LugoFs solution the grain-residues all color 

 a light or a light moderate indigo; the capsules a red 

 or a reddish violet, and the solution a very deep indigo, 

 as in both parents. 



Quantitatively the reaction with iodine is the same as 

 in N. gloria muudi; qualitatively there are no noticeable 

 differences between the hybrid and the parents. 



Aniline Reactions. 



With gentian violet the grains all color very lightly at 

 once, and in half an hour they are light to moderately 

 colored (value 35), midway between N. gloria mundi and 

 N. poeticus ornatus. 



With safranin the grains all color very lightly at once, 

 and in 30 minutes they are all light to moderately colored 

 (value 40), the same as in N. gloria mundi. 



In the reaction with aniline stains N. fiery cross shows 

 with safranin a closer relationship to N. gloria mundi 

 than to N. poeticus ornatus. With gentian violet it is 

 midway between the two parents. 



Temperature Reactions. 



The temperature of gelatinization of the majority 

 of the grains is 71° to 72° C, and of all is 73.5° to 

 75.5° C, mean 74° C. 



The temperature of gelatinization of N. fiery cross 

 is closer to that of N. gloria mundi (mean 74.5°) than to 

 that of N. poeticus ornatus (mean 77.5° C). 



Effects of Various Reagents. 



The reaction with chloral hydrate begins in a few 

 grains in 2 minutes. Complete gelatinization occurs in 

 about 0.5 per cent of the entire number of grains and 

 total starch in 5 minutes; in about 1 per cent of the 

 grains and 3 per cent of the total starch in 15 minutes ; 

 in about 2 per cent of the grains and 5 per cent of 

 the total starch in 30 minutes; in about 5 per cent of 

 the grains and 9 per cent of the total starch in 45 

 minutes; in about 7 per cent of the grains and 13 per 

 cent of the total starch in GO minutes. (Chart D 287.) 



The hilum in distinctness and in the size of the 

 bubbles formed is the same as in N. gloria mundi, but 

 the latter appear in more grains than in N. gloria mundi, 

 and in many more than in N. poeticus ornatus. The 

 lamellae, as in the parents, are at first indistinct, but 

 later are as distinct as in N. gloria mundi. The grains 

 become more refractive, the first part of the grain to 

 show this change is a rather narrow marginal band, 

 which is as narrow and as refractive as in N. gloria 

 mundi. Gelatinizaton begins usually at one or two 

 points at the distal margin, rarely at so many different 

 points as in either parent. The progress of gelatiniza- 

 tion is very similar to that described under N. gloria 



mundi, only it is smoother, more even in all parts of the 

 grain, and accompanied by less Assuring than in that 

 starch — accentuations of characteristics of A. gloria 

 mundi. 



The gelatinized grains are much swollen, as in the 

 parents, and have as thin capsules and are as much dis- 

 torted as in N. gloria mundi. 



In this reaction N. fiery cross shows qualitatively 

 a much closer relationship to N. gloria mundi than to 

 N. poeticus ornatus. 



The reaction with chromic acid begins in 1 minute. 

 Complete gelatinization occurs in less than U.5 per cent 

 of the entire number of grains and 3 per cent of the 

 total starch in 5 minutes ; in less than 0.5 per cent of the 

 grains and 12 per cent of the total starch in 15 minutes; 

 in about 5 per cent of the grains and GO per cent of 

 the total starch in 30 minutes; in about 25 per cent of 

 the grains and 85 per cent of the total starch in 45 

 minutes; in about Go per cent of the grains and 95 per 

 cent of the total starch in 60 minutes. (Chart D 288.) 

 (See footnote, page 516.) 



The hiluni is as distinct as in N. gloria mundi, but 

 not so distinct as in N. poeticus ornatus, and the lamella? 

 are never so distinct as in either parent. Gelatinization 

 begins at the hilum and in a great majority of the grains 

 progresses according to the method described under N. 

 gloria mundi, the process being very nearly the same, 

 except that there is more extensive and deeper fissuring 

 of the part of the grain which is included between the 2 

 transverse furrows or fissures which are projected from 

 the side of the hilum ; and the striae seen in the proximal 

 deposit are coarser and more distinct. A rather small 

 minority follows the method described for the majority 

 of the grains of N. poeticus ornatus. The gelatinized 

 grains are much swollen, have as thin cajisules, and are 

 no more distorted than in N. gloria mundi. In this 

 reaction N. fiery cross shows qualitatively a much closer 

 relationship to N. gloiia mundi than to N. poeticus 

 ornatus. 



The reaction with pyrogallic acid begins in 1 minute. 

 Complete gelatinization occurs in about 0.5 per cent of 

 the entire number of grains and 3 per cent of the total 

 starch in 5 minutes; in about 3 per cent of the grains 

 and 23 per cent of the total starch in 15 minutes ; in about 

 28 per cent of the grains and 70 per cent of the total 

 starch in 30 minutes; in about 43 per cent of the grains 

 and 86 per cent of the total starch in 45 minutes; in 

 about 54 per cent of the grains and 92 per cent of the 

 total starch in 60 minutes. (Chart D 289.) 



The hilum and lamella? are as distinct as in N. gloria 

 mundi. Gelatinization begins at the hilum and pro- 

 ceeds according to two methods. In the great majority 

 of the grains the method is that described for all the 

 grains of N. gloria mundi, and with certain modifications 

 for a minority of the grains of N. poeticus ornatus. In 

 a small minority it is the same as that described for a 

 majority of the grains of N. poeticus ornatus, except 

 that the striaa which divide the grains are not so fine and 

 the lamellated appearance in the marginal band is more 

 persistent. 



The gelatinized grains are as much swollen, have as 

 thick capsules, and are as much distorted as in the 

 parents. 



