782 



DATA OF PROPERTIES OF STARCHES OF PARENT- AND HYBRID-STOCKS. 



much lower than in C. vestita var. rubro-oculata, but 

 considerably higher than in the other parent. There is 

 a variation both in the sides of the box arrangement 

 described under figure and in the grains with entire 

 polarization as in both parents. 



With selenite the entire quadrants are moderately 

 often sharply defined, much less frequently than in C. 

 vestita var. rubro-oculata, but considerably more often 

 than in the other parent ; when present, they vary as in 

 both parents. The colors are more frequently impure, 

 much less pure than in C. vestita, var. rubro-oculata, 

 but considerably more than in the other parent. The 

 impurity is at both ends of the scale as in parents. 



In degree of polarization, character of the figure, 



and appearance with selenite the grains of C. bryan 



are closer to those of C. regnieri than to C. vestita var. 



rubro-oculata. 



Iodine Reactions. 



With 0.25 per cent LugoFs solution the grains imme- 

 diately color a moderate violet (value 38), almost as deep 

 as in C. vestita var. rubro-oculata, and a little deeper 

 than in the other parent ; they deepen quickly, becoming 

 bluer in tint with considerable variation in depth of the 

 different grains; the main body of the grain is often 

 deeper than the margin, which may be almost colorless. 

 The grains become neither so deep nor so bluish in tint as 

 in C. vestita var. rubro-oculata, and there is also a 

 greater variation among the different grains and in the 

 same aspect of a given grain than in C. vestita var. 

 rubro-oculata. The grains deepen with about the same 

 variation in the different grains, with slightly less in 

 the same aspect of a given grain, and become a little 

 more bluish in tint than in C. vestita var. rubro-oculata. 

 With 0.125 per cent Lugol's solution the grains color a 

 light violet which quickly deepens, becoming bluer in tint, 

 the variations in the different grains and the same aspect 

 of a given grain are about as above, the comparison with 

 the parents is the same as with the 0.25 per cent Lugol's 

 solution. After heating in water until the grains are 

 gelatinized and then adding a 2 per cent Lugol's solution, 

 the gelatinized grains become light to moderate blue 

 with a very reddish tint; they are more reddish and 

 lighter than those of G. vestita var. rubro-oculata, but 

 are less reddish and there is less variation in depth with 

 mean slightly deeper than in C. regnieri. The solution 

 is a deep blue, deeper and less greenish in tint than in 

 both parents. If the preparation is boiled for 2 minutes 

 and then treated with an excess of 2 per cent Lugol's 

 solution, the grain-residues color a light blue with reddish 

 tint, about the same depth as in both parents, a little more 

 reddish than in C. vestita var. rubro-oculata, but hardly 

 so reddish as in C. regnieri. The capsules color a deep 

 old-rose to either wine-red or a deep heliotrope. The 

 color has a little more yellow in the red than in C. vestita 

 var. rubro-oculata, but not nearly so yellowish as in C. 

 regnieri; it is deeper than in both parents. The solution 

 is a very deep blue, deeper than in both parents. In the 

 qualitative reactions with iodine the grains of C. bryan 

 are closer to those of C. vestita var. rubro-oculata, than 

 to those of C. regnieri. 



Aniline Reactions. 

 With gentian violet most of the grains color lightly, 

 while a few become a moderate violet; more variation 



with the mean deeper than in C. vestita var. rubro-ocu- 

 lata; but there being less of the moderate, the mean is 

 not as deep as in C. regnieri. In half an hour they 

 become moderate to moderately deep (value 53), with a 

 larger proportion of the former, the mean a little lighter 

 than in C. vestita var. rubro-oculata; and less variation in 

 depth with the mean deeper than in C. regnieri. The 

 variation in depth of the main body and the margin in 

 the same aspect of a given grain is much more frequent 

 than in C. vestita var. rubro-oculata, but not so marked 

 nor quite so frequent as in C. regnieri. The variation 

 in depth of the rare fissures and body of the grain is less 

 marked than in C. vestita var. rubro-oculata, but more 

 than in C. regnieri. 



With safranin most of the grains immediately become 

 light to moderate ; about the same depth as in C. vestita 

 var. rubro-oculata, a little lighter than in C. regnieri. 

 In half an hour they stain moderate to moderately deep 

 (value 63) ; the variation in the depth of the same 

 aspect of a given grain is the same as with gentian violet ; 

 a triile lighter, than in C. vestita var. rubro-oculata; 

 deeper and less of yellow in the red than in C. regnieri. 



In the reactions with aniline stains the grains of C. 

 bryan are slightly closer to C. regnieri than to C. vestita 

 var. rubro-oculata. 



Temperature Reactions. 

 The temperature of gelatinization for the majority 

 of grains is at 72° to 74° C, and of all but rare resistant 

 grains at 76° to 77.5° C, mean 76.7° C. At the 

 beginning of heat gelatinization, the steps more closely 

 resemble those of C. regnieri, but in the later stages the 

 outermost lamella? are gelatinized and become convoluted 

 as in C. vestita var. rubro-oculata, so that the gelatinized 

 grain closely resembles this species, instead of 0. regnieri. 

 The temperature of gelatinization of 0. bryan is much 

 closer to C. regnien than to C. vestita var. rubro-oculata. 



Effects of Various Reagents. 



The reaction with chloral hydrate begins immediately. 

 Complete gelatinization occurs in about 29 per cent of 

 the entire number of grains and 61 per cent of the total 

 starch in 5 minutes; in about 48 per cent of the grains 

 and 75 per cent of the total starch in 15 minutes ; in about 

 74 per cent of the grains 'and 89 per cent of the total 

 starch in 30 minutes ; in about 79 per cent of the grains 

 and 91 per cent of the total starch in 45 minutes; in 

 about 89 per cent of the grains and 94 per cent of the 

 total starch in 60 minutes. (Chart D 627.) 



The hilum becomes distinct in some of the grains and 

 a bubble is frequently formed there, also deep fissures 

 occur which during gelatinization divide the grain into 

 several parts. The lamella} become distinct in some of 

 the grains as in C. vestita var. rubro-oculata, but in others 

 there is no trace of either hilum or lamellae, as they 

 resemble the characteristic grains of C. regnieri. A nar- 

 row refractive band forms slowly about the margin of the 

 grain and recedes as gelatinization advances from the 

 margin, as in C. vestita var. rubro-oculata, Gelatiniza- 

 tion begins in the interior of some of the grains as in 

 C. regnieri, but in most at the margin, or at the margin 

 and at the hilum as in C. vestita var. rubro-oculata, and 

 the process is the same as in G. vestita var. rubro-oculata. 

 The gelatinized grains have rather thin capsules, are 



