MACROSCOPIC AND MICROSCOPIC CHARACTERS OF PLANTS. 



833 



dots over the veins. Under the microscope coloration is 

 found to be due to the same causes as in 0. villosum, 

 except that the clusters of cells containing red-violet sap 

 consist of fewer cells, are less numerous, and further 

 apart. In the hybrid the area is pale yellowish green, 

 with purple hairs, and red purple dots over the veins, the 

 color being deeper than in C. villosum, and the area larger 

 than in G. insigne maulei. The appearance under the 

 microscope is similar to that in G. insigne maulei, but 

 there is a greater number of cells in the area that contain 

 red-violet sap. 



Sections of the inner epidermis at the most anterior 

 part of the labellum along the mid-line were examined in 

 the three plants. The upper epidermal tells are very 

 wavy-walled and smaller in C. villosum than in C. insigne 

 maulei, and larger in the hybrid than in either parent. 

 (Table J 61.) 



Long hairs are present in this region. They are 

 shorter in G. villosum than in G. i7isigne maulei, and 

 shorter in the hybrid than in either parent. (Table J 61.) 



The color in C. villosum is pale brownish-greenish- 

 violet, with purple hairs, due to many cells being filled 

 with lavender sap and yellow chromoplasts, and to a 

 few cells with yellow chromoplasts only. Hairs mostly 

 contain a pale- violet sap and yellow chromoplasts ; a few 

 contain orange or brown chromoplasts only. In C. in- 

 signe 7naulei the color is yellowish brown, with purple 

 hairs, due to the cells containing yellow chromoplasts, 

 the hairs containing yellow chromoplasts and a pale- 

 violet cell sap, or with orange and orange-brown chromo- 

 plasts only. In the hybrid the color is brownish-greenish- 

 violet, darker than in C. villosum, with purple hairs, due 

 to many cells containing a lavender sap and yellow 

 chromoplasts, and to a few cells with yellow chromoplasts 

 only. Hairs containing a pale-violet sap and yellow 

 chromoplasts are more numerous than in V. insigne 

 maulei, and only a few contain only orange or brown 

 chromoplasts. 



Sections of the lower epidermis of the labellum were 

 taken between the apex and the most anterior point 

 (between the top of the slipper and the toe). The cells 

 are all large and very wavy-walled. They are a little 

 smaller in C. villosum than in C. insigne maulei, and 

 larger in the hybrid than in either parent. (Table J 61.) 



The color of this region in G. villosum is brownish 

 green with brownish-violet veins, due to some cells con- 

 taining only yellow chromoplasts, and to others with a 

 pale-lavender sap and yellow chromoplasts. In G. in- 

 signe maulei the color is yellow with brownish-violet 

 veins, darker than in G. villosum, and due to a pale- 

 lavender cell sap and yellow chromoplasts in some cells, 

 and to yellow chromoplasts only in other cells. In the 

 hybrid the color is brownish green with brownish-violet 

 veins, very similar to G. villosum, but darker, the brown- 

 ish-violet veins being not so dark as in G. insigne maulei; 

 the cell sap is darker than in either parent, and nearer a 

 red-violet than a lavender. Some of the cells contain 

 both yellow chromoplasts and colored sap, and others 

 only yellow chromoplasts. 



Sections of the lower epidermis were also taken at the 

 base of the labellum along the mid-line. The cells are 

 large and have thin, wavy walls. They are smaller in 

 C. villosum than in C. insigne maulei, and larger in the 

 hybrid than in either parent. (Table J 61). 



The color of this area in C. villosum is yellowish green 

 (more greenish than in the hybrid), due to the presence 

 of a few pale-yellow chromoplasts in the cells; in C. 

 insigne maulei greenish yellow; in the hybrid greenish 

 yellow (more yellowish than in C. insigne maulei), to 

 yellow chromoplasts in the cells. 



Comparative Summary of the Characters of the 

 Hybrid Cypripedium nitens and its Parents. 

 The hybrid was found to be : 

 (1) The same or practically the same as the seed 



parent: In the color of the rloweT-stalk ; length of the 



