434 CONNECTICUT EXPERIMENT STATION REPORT, IQI2. 



parasitica will grow in higher per cents, of tannic acid and give 

 a more evident development of mycelium than E. gyrosa. The 

 details of this experiment are given in the appended table. 



INOCULATION EXPERIMENTS. 



General Conditions, etc. These experiments were undertaken 

 primarily to determine the parasitic tendency of Endothia gyrosa 

 as compared with that of the variety parasitica. That the latter 

 could produce cankers when inoculated into chestnuts had been 

 abundantly proved by the work of Murrill and others. With 

 most of our inoculations both the species and the variety were 

 used at the same time, and checks were also included. Nearly 

 all these inoculations were made from artificial cultures, and 

 usually only with conidial spores. Ordinarily a small slit in the 

 bark was made with a sharp scalpel, spores from the cultures 

 were introduced on a needle, the wound covered with moist 

 cotton, and then bound with paraffine paper or bicycle tape. 

 After several weeks the covering was removed. The checks 

 were treated in the same way, except that no spores were intro- 

 duced into the wound. 



In this way there were inoculated two- to three-year-old 

 seedling chestnuts, four- or five-year-old chestnut sprouts, and 

 two-year seedling oak at the Station Farm at Mount Carmel; 

 six- to eight-year-old slow-growing chestnut seedlings at the 

 Station forestry plantation at Rainbow; and two- to four-year- 

 old oak sprouts in a waste lot at Highwood. The tables which 

 follow give the data for all inoculations, since there are factors 



