62 BULLETIN 380, U. S. DEPARTMENT OF AGRICULTURE. 



ENDOTHIA SINGULARIS. 



The material of Endothia singular'^ distributed by Sydow as Calo- 

 pactis singularis was on Quercus gambellii Nutt. The writers have 

 seen abundant material on this species as well as specimens on Q. 

 utahensis (A. DC.) Rydb., Q. leptophylla Rydb., and Q. nitescens 

 Rydb. Specimens on the latter two hosts were sent by Bethel, who, 

 in a letter, reports finding this species also on Q. pungens Liebm. 



All of these species except Quercus leptophylla are chaparral- 

 forming shrubs growing at an elevation of 4,000 feet or more. 

 There is at present no evidence that the fungus is parasitic on any 

 of the species. 



Inoculations with the mycelium of Endothia singularis were made 

 on Fagus and on Quercus alba, Q. velutina^ Q. rubra, and Q. palustris, 

 as well as on Q. Uidfolia on Overlook Mountain in the Catskills. 

 No growth has, however, been noted in any case. 



ENDOTHIA FLUENS. 



When these investigations were commenced, the writers thought 

 that the Endothia found in Europe might be the same as Endothia 

 parasitica found in America. Inoculations were accordingly made 

 in Maryland during October, 1912, with cultures from material col- 

 lected on the chestnut by the senior writer at Stresa, Italy, and 

 Etrembieres, Switzerland, using material of E. fluens sent by P. J. 

 Anderson from Pennsylvania; also material of that species and of 

 E. parasitica collected in Virginia as checks. In this case, as in all 

 others where no special mention is made of the method, inoculations 

 were made by cutting through the bark to the wood with a sharp 

 knife. The inoculating material was then inserted with a freshly cut 

 twig and the wound tied up either with cord or rubber bands. If 

 cord was used it was cut away within two to four weeks. The rub- 

 ber bands became loosened by exposure to the weather within about 

 the same time. 



Inoculations were made with all the above material on sprouts of 

 Castanea dentata and Quercus prinus. The results are summarized 

 in Table IV. 



TABLE IV. Inoculations of Endothia in Maryland in October, 1912. 



