Fifty grams: Peas, beans, corn, lupines, cotton, and cowpeas. 



Amounts to be taken of seeds not enumerated shall be the same as 

 those required for seeds named which are of similar size. 



In case the sample is suspected to contain any seed of a pest, like 

 dodder, Canada thistle, wild mustard, plantain, etc., at least 50 grams 

 shall be examined for said impurity. 



(3) Germination tests (a) Se<d. Seed for germination tests is to be 

 taken indiscriminately from pure seed which has been thoroughly mixed 

 for that purpose. One hundred seeds of peas, beans, corn, cucurbits, 

 and those of a similar size, and 200 seeds of clover, spinach, Cruciferse, 

 and others of similar size and smaller shall be taken for each single test. 



(b) Duplicate tests and allowable variation. The laboratory tests 

 shall be made in duplicate simultaneously, under identical conditions, 

 and the average result taken. If the duplicated tests vary more than 

 10 per cent, they shall be repeated; also a supplementary test should 

 be made in sand. 



(c) Substratum to hold seeds. For a substratum or seed bed the 

 committee recommends for the present year a blue blotting paper, to be 

 obtained of Carter, Eice & Co., 246 Devonshire street, Boston, Mass. 



(d) Hard seeds. At, the close of the blotter test one-third of the 

 leguminous seeds which remain hard shall be counted as viable. 



(e) Supplementary tests. We recommend supplementary tests in sand 

 whenever practicable in the case of the Poas, Agrostis spp., celery, 

 tobacco, and all seeds which in laboratory tests fall 10 per cent or more 

 below the germination standard adopted by the station. 



Seeds of Agrostis, Poa, yellow oat grass, tobacco, and others of a 

 similar size are to be sown upon the surface and the lightest possible 

 covering of sand given them. Other seeds are to be planted at depths 

 about equal to twice their diameter. All seeds are to be planted far 

 enough apart to avoid contact during the process of germination. 



The supplementary tests shall be made at a temperature of 20 to 

 30 C. (68 to 86 F.) in sand sterilized by heating, free from organic 

 matter, and sifted to secure a uniform size. Sieves with a mesh of 1 

 millimeter (one-twenty-fifth inch) are recommended for this purpose. 



In sand tests only those seeds shall be counted viable whose sprouts 

 appear above the surface of the ground. The results of the supple- 

 mentary tests shall be accepted when they show a higher percentage 

 than those in blotters; otherwise the percentages secured in blotter 

 tests only shall be used. 



(f) Moisture. The sand and blotters shall be kept well moistened, 

 but not saturated during the germination tests. Only potable water 

 of a temperature approximating that of the seed bed shall be used. 



(g) Duration of the germination tests. The following periods of time 

 shall be used for blotter tests: Ten full days for cereals, spurry, peas, 

 beans, vetches, lentils, lupines, soja beans, sunflower, buckwheat, Cru- 

 ciferae, Indian corn, and cowpeas; 14 full days for serradella, espar- 

 sette, beet u balls," rye grass, timothy, Umbelliferse, tobacco, Lespedeza, 



