and all other field and vegetable seeds not herein enumerated; 21 full 

 days for grasses except. Poa, Bermuda grass, rye grass, and timothy; 

 28 full days for Poa and Bermuda grass. 



Each day the seed sprouted in blotters should be counted and re- 

 moved and a careful record made of the same. Sand tests are to be 

 continued two days longer in each case and the sprouts counted only at 

 the close. 



(h) Temperature in germinating tests. It is recommended that the 

 temperature be kept at 20 0. for eighteen hours out of each twenty- 

 four, and in no case shall it fall below 15 0. or rise above 32 0. 



For six hours out of each twenty-four the tests of all grasses (except 

 fescues, rye grasses, and cereals) and cucurbits, cotton, eggplant, 

 tomato, pepper, and Lima bean should be raised to 30 0. The tests 

 of all other seeds not requiring high temperatures should be lowered to 

 16 to 18 0. for the same period. 



(i) Germinating chamber. The germinating chamber may be of any 

 form which gives the operator proper control over the conditions of 

 light, heat, air, and moisture. We recommend the form which is now 

 in use in the seed laboratory of the United States Department of Agri- 

 culture, marked "Standard," and made by Ernest Betz, Washington, 

 D. 0. Said chamber is to be provided with a low-temperature thermo- 

 regulator. 



(4) Keeping samples. A sufficient amount of each sample should be 

 kept by the station in well-corked vials in a dark, dry, and cool place 

 for six months, to be used in case a retest is found necessary. 



(5) Record. The report of seed tests shall include name of station, 

 station number, name of seed, source of sample, weight of sample, date 

 of tests, percentage by weight and character of impurities, kind of 

 seed bed, temperature of seed bed, number of sprouts germinated each 

 day, percentage germinated, and number of hard seeds which remain at 

 close of test. 



We recommend the form of record blank annexed, No. 1. 



(6) Report blank. Form No. 3 is recommended for reports of complete 

 tests. 



(7) Time for making germination tests. Purchasers of guaranteed seed 

 shall send in their samples not later than fifteen days from the date 

 of the receipt of said seed by them. All germination tests shall be 

 commenced within fifteen days after the seed has been received by the 

 station. 



(8) Accessory apparatus. (a) A chemical balance weighing up to 100 

 grams and sensitive to 1 milligram, kept in a case, together with accu- 

 rate metric and avoirdupois weights. 



(b) A standard simple dissecting microscope and a large reading glass 

 or pocket lens. 



(c) Botanical forceps and dissecting implements. 



(d) An authentic collection of the seeds of the principal weeds and 

 economic plants. 



