CHANGES ACCOMPANYING BREAKING OF REST PERIOD 3 



for a few isolated areas in the internodes which show the reddish 

 brown tint. These areas of normal color are confined to the inter- 

 nodes nearest the terminal buds. A few of the untreated twigs are 

 beginning to turn yellow on the bud scales and bud shoulders. All 

 internodes are reddish brown in color. After eight days about one- 

 fourth of the surface of untreated twigs is still reddish brown in the 

 internodes. All etherized twigs are yellow thruout. They have 

 been so since the fourth day." 



These experiments indicate that etherization tends to hasten 

 changes which normally occur within the twig. 



3. Amount of Sugar and Growth. Jonathan apple twigs 

 were collected on February 8, 1914. Some were etherized twenty- 

 four hours; some remained untreated. The twigs remained in the 

 respiration chamber for three days after which some etherized and 

 some untreated twigs were placed in the greenhouse in beakers con- 

 taining 50 cc. water. The cuts on the bases of the twigs were re- 

 newed every day so they would take up water readily. 



After four days, the buds on the etherized twigs began to unfold ; 

 those on the untreated twigs remained normal. Sugar determina- 

 tions made at this time (using the methods previously described) 

 showed that the etherizing did not increase the sugar content. After 

 a few more days the buds of the untreated twigs opened and by the end 

 of the fourteenth day the leaves had outgrown those on the ether- 

 ized material. This time the sugar determinations indicated that 

 the normal twigs had the larger amount of sugar. After another 

 four or five days the leaf growth in all twigs ceased. Again the rela- 

 tive amounts of sugar in both treated and untreated twigs was deter- 

 mined, both sets showing similar amounts. 



4. Respiration and Growth. Jonathan apple twigs were col- 

 lected on February 9, 1914. Some lots were etherized for twenty- 

 four hours; some remained untreated. After this preliminary treat- 

 ment, all were put in the respiration chamber. During the first few 

 days the etherized twigs gave off more CO2 than untreated twigs. 

 During this period, and on the second day, some of the treated and 

 some untreated lots were removed from the respiration chamber 

 and, after clipping off the bases of the twigs, they were placed in the 

 greenhouse in beakers containing water. The twigs were allowed 

 to grow for a period of four days after which they were immersed 

 in a strong formalin solution, in order to stop further growth, and 

 at the same time preserve the general appearance of the growth 

 already made. After the fourth day, the CC>2 production of the 

 etherized material had fallen below that of the untreated. (Cf. curve 



