APPENDIX. 



Collection and Preparation of Material. 



Amoeba. While amoeba has a wide distribution, -in no 

 place is it very abundant, nor are culture methods as success- 

 ful as with other protozoa. By putting Elodea, Cerato- 

 phyllum, or other water plants, into a shallow dish with a 

 small amount of water and allowing the plants to decay, 

 amoebae may often be found in some abundance in the slimy 

 sediment. The slime on lily pads often contains amoebae. 

 Occasionally amoebae will be extremely abundant in the 

 scum on a freshly started hay infusion, especially when 

 pond weeds have been present in the infusion. 



Par amecium. Into a hay infusion twenty-four hours old 

 (that made from dry timothy hay is best), place some water 

 and organic matter from almost any pond or swamp. After 

 about a week Paramecium will be found in abundance. Or 

 fill a jar half full of Elodea or other pond weeds, cover with 

 water and allow the plants to decay. Do not place the 

 jars in the direct sunlight. 



To keep a culture in vigorous condition remove some of 

 the old hay and about one-third of the water, and add fresh 

 hay and water, every two or three days. If several cultures 

 are running and are changed on different days one may have 

 vigorous cultures of Paramecium constantly. 



Conjugating paramecia are often obtained a few days or 

 a week after a fresh culture is started, if the culture has been 

 started by bringing in Paramecium from outside. 



