APPENDIX 157 



in 100 cc water and make up to 150 cc with water. Add 

 the cupric sulphate solution to the carbonate-citrate solution 

 slowly, stirring. The mixed solution is ready for use. 



Borax Carmine. Dissolve 4 g. borax in 100 cc water. Add 

 1 g. carmine and dissolve it with heat. Cool and add to 

 the solution 100 cc of 70 per cent alcohol. Filter after 

 twenty-four hours. A good stain for large objects and for 

 tissues in bulk. Use the stain twenty-four hours, and 

 differentiate with acid alcohol. 



Bouin's Fluid. 



Saturated aqueous picric acid 75 cc 



Commercial formalin 25 " 



Glacial acetic acid . 5 " 



It is best to add the acetic acid just before using. Kill 

 the tissue four to twenty-four hours, wash in 50 per cent 

 alcohol, preserve in 80 per cent alcohol. This is one of the 

 best of the killing fluids for general use. 



Chloretone. Make a. saturated solution in water. Used 

 for narcotizing animals. In some cases it may be sprayed 

 on top of the water which contains the animals. For nar- 

 cotizing frogs for demonstrating the circulation of the blood 

 in the web the following method has proved satisfactory: 

 With a pipette inject about 2 cc into the stomach through 

 the esophagus. If after twenty minutes the animal is not 

 quiet repeat the dose. The animal will remain quiet for 

 hours, but will be recovered within twenty-four hours. 



Chloriodide of Zinc. 



Chloride of zinc 30 . g. 



Potassium iodide 5 . g. 



Iodine 0.89g. 



Dissolve the above ingredients in about 15 to 20 cc distilled 

 water. The solution does not keep long, and should be 

 kept in the dark. Used as a test for cellulose. 



