APPENDIX 159 



color. Now counterstain the sections, if desired, dehy- 

 drate, clear and mount. 



Injection Mass. The following is one of the best masses 

 for injecting bloodvessels. 



Dry corn starch 1 Ib. 



Chloral hydrate (2 per cent) 600 cc 



Alcohol (95 per cent) 150 " 



Color 150 " 



The chloral hydrate and alcohol of the above may be re- 

 placed by 750 cc of 5 per cent formalin; the resulting mass 

 appears to act as well as the one above. To make the 

 color take about 50 g. of dry insoluble color, such as chrome 

 yellow or vermilion, grind in a mortar with 50 cc of glycerin 

 and 50 cc of alcohol (95 per cent). Mix the color and the 

 liquid and slowly stir in the starch to make a homogeneous 

 mass. If the mass appears to be getting too thick use less 

 starch, if too thin add more starch. Before using the mass 

 should be strained through two thicknesses of cheesecloth, 

 to remove all particles which might clog the cannula or 

 artery. The injection mass does not spoil upon standing, 

 but must be well stirred before using. 



Iodine Solution. Dissolve potassium iodide in distilled 

 water to saturation, then saturate this solution with metallic 

 iodine. To use dilute with several volumes of water. May 

 be used as a stain for protoplasm, but chiefly used for starch 

 test. 



Lime (or Baryta) Water. Shake up a little quick lime 

 (or barium oxide for baryta water) in water and allow the 

 mixture to settle. Decant or filter the clear liquid. In the 

 presence of carbon dioxide this clear liquid will become 

 milky, or will show a white precipitate. 



Lyons Blue. This is one of the anilin dyes. An alcoholic 

 solution should be made. This is used as a contrast stain 

 with borax carmine. 



