xii INTRODUCTION TO SECOND EDITION. 



Many of Professor Brooks's students have carried 

 on scientific investigations of the life of the oyster, and 

 three have been called upon for practical work in Mary- 

 land and elsewhere. 



In 1896, Dr. H. McE. Knciwer was employed to 

 make a biological examination of a site for an exten- 

 sive oyster farm, and to report, to those who were 

 interested, the results of his examination. 



In 1899, Dr. Caswell Grave was employed by the U. 

 S. Fish Commission and by the Geological Survey of 

 North Carolina to make a biological examination of 

 the oysters and oyster beds of North Carolina, and, 

 later, to carry on experiments in oyster culture, and to 

 establish an experimental oyster farm. Dr. Grave has 

 devoted three years to this work, and the oyster beds 

 which he has established in the vicinity of Beaufort, 

 N. C., are now open to the public and are supplying 

 oysters for the market. An account of his investiga- 

 tions and experiments was published in 1904. 



Dr. O. C. Glaser, who assisted Dr. Grave in his ex- 

 periments, was employed in 1903 by the Geological 

 Survey of Louisiana to take charge of the Gulf Bio- 

 logical Station, and to carry on experiments in oyster 

 culture in the Gulf of Mexico. An account of his ex- 

 periments was published in 1904. 



IRA REMSEN. 



Office of the President of the 

 JOHNS HOPKINS UNIVERSITY, 

 March 25, 1905. 



