THE DEPARTMENT TREES. 23 



putable evidence that the disease can be spread in this way, although it 

 seems very probable. Several extensive experiments of this kind are 

 now in progress. In experiments 1 and 2, in all or almost all of the 

 inoculations there was a distinct union between the bud and the stock, 



1. e., between the diseased and healthy tissues, some months prior to 

 the first appearance of symptoms in the stocks. 



Experiment 5. In order to be absolutely certain as to the healthy 

 character and sound constitution of the seedlings, stones were pro- 

 cured in quantity from three localities free from yellows. These 

 were planted November 8, 1889, upon well drained and fertile soil in 

 the grounds at the Department of Agriculture. Row No. 1 were Ten- 

 nessee pits. "These pits were bought of Johnson & Stokes, Philadel- 

 phia, and came from London, Tennessee." I procured them from Charles 

 Wright, Seaford, Delaware. Eow No. 2 came from Sussex County, Dela- 

 ware, and were also procured, from Mr. Wright, who had purchased 

 them for his own use. Concerning them he wrote : "The Delaware 

 pits were collected by myself of William P. Brown, of Seaford, and 

 were saved from old seedling trees in his orchard near town." Row 

 No. 3 came from Caroline County, Maryland. They were collected by 

 J. W. Kerr, of Denton, for his own use. 



The seedlings came up satisfactorily and made a good growth in the 

 season of 1890. About 100 of each row were inoculated and the rest 

 were reserved for future experiments. 



The buds for inoculation were selected with the greatest care from 

 17 diseased trees in the older orchard of James W. Green, Magnolia, 

 Delaware (No. 14 of first report). I did not have an orchard map 

 when the buds were cut, but, judging from the appearance of the 

 trees, all were cases of 1889. Each one was plainly diseased by yel- 

 lows, but none were wholly diseased, i. e., none appeared to be so. 

 One-third .to one-half of each tree was more or less yellow and bore 

 the diseased shoots, but the other side was green and thrifty and although 

 examined very minutely gave no indication of disease. Two shoots 

 were taken from each tree (different limbs). These were 2 to 3 feet long, 

 robust, well matured for the time of year, and to all appearances per- 

 fectly healthy. Each shoot came from the healthy-looking side of the 

 tree, and all bore smooth, green, and vigorous leaves, 6 to 9 inches long. 

 Better looking buds were never used. These shoots grew out at much 

 greater distances from diseased parts than those used for Experiment 



2. They were, cut July 18, 1890, immediately stripped of foliage, and 

 wrapped in damp cloths. Row 1, was budded July 19; row 2, July 21; 

 and row 3, July 22. All of the buds were used except some on the 

 base of the shoots which proved too large for the stocks. Two buds 

 were put into each seedling, but owing to dry weather and unskillful 

 manipulation, many of them failed to unite with the stock. Others 

 healed on satisfactorily but made no growth. Still others made a 

 growth of 1 to 10 inches, although the tipper portion of the stocks was 

 purposely left uncut in order to prevent this. 



