RESULT OF INOCULATIONS. 51 



(2) Two trees were badly diseased, but a few small branches bad developed normal, 

 or nearly normal, leaves and shoot-axes, while a few others bore sickly yellowish 

 leaves and no rosettes. These trees were dwarfed, and at least nineteen-twentieths 

 of the branches bore yellowish green rosettes. 



(3) The remaining trees, 113 in number, were diseased in all parts, exactly as 

 had been the trees from which the buds were taken. The leaves and shoot-axes 

 were stunted, yellowish green, and bunched into striking rosettes. Out of this 

 large number of trees there was not one which bore a single healthy leaf or a nor- 

 mally-developed shoot-axis. Every part of each tree was completely diseased. The 

 greater number were still alive in all parts, but a few were withering (see left-hand 

 figure of plate xxxv), and a few were already dead. Almost all of the winter buds 

 developed into rosettes in spring, and in some cases these appeared also on the stems 

 above and below the location of the inserted buds (plate xxxv). 



A more satisfactory outcome could scarcely be desired for any ex- 

 periment whatsoever. 



Each tree was carefully examined, and notes were made on the con- 

 dition of each inserted bud, These observations were also verified, at 

 my request, by Mr. W. T, Swingle, of the Division of Vegetable Path- 

 ology, so as to leave no doubt whatever concerning their value. 



In the four trees which remained healthy the inserted buds failed to 

 unite with the stocks and were thrown off. the bark of the stock hav- 

 ing healed over under them. The only uncertainty was concerning a 

 small fragment of the bark of one bud, and the evidence of union in 

 this case was so unsatisfactory that it was entered as "very doubtful.' 7 

 Three of these trees received two buds each, but by accident the other 

 received only one bud. 



In one of the 1.15 diseased trees the evidence of union between the 

 stock and the two inserted buds was also doubtful. In all of the others 

 some part of one or both buds had united with the stock, and in many 

 instances was still living. This was also true of the 6 trees removed 

 in 1890. Often, however, only small fragments of each bud healed on. 

 Occasionally less than one-fourth of 1 square centimetre of the bark of 

 one bud was all of the diseased tissue that had actually united with 

 the stock, and yet this was sufficient to induce the disease. None of 

 the inserted buds pushed over 3 inches, most failed entirely or pushed 

 only one-fourth inch to 1 inch and then died. 



The diseased seedlings were noticeably smaller than the four healthy 

 ones. On an average they were about 3 feet high with a proportionate 

 spread of branches. 



The contrast between the November and June examinations is note- 

 worthy. In November, when the disease had been acting less than 

 5 months, only 66 of the stocks showed any symptoms whatever. 

 None of them were wholly diseased; most showed no signs of disease 

 at a distance, and even a large part of the six worst cases was still free 

 from symptoms (plate xxxiv). In June, after the disease had been 

 acting nearly a year, 121 of the original 125 trees had become affected, 

 and 115 so completely that it was almost impossible to find any healthy 

 branches. The only uncertainty is concerning the six which were 

 removed in November for photographic purposes. Their condition, 



