RESULT OF INOCULATIONS. 53 



green, and their buds showed no tendency to germinate. Moreover, all 

 of the foliage on the branches in the immediate vicinity was full grown 

 and healthy, and there were no signs of disease nearer than the base of 

 the main branches, 4 to 6 feet below. The buds were cut July 1, 1890, 

 and inserted the same day two into each tree in the usual way. A 

 subsequent examination showed that many of them had healed on and 

 were living. The tops of the cuttings were not removed until some 

 weeks later. 



These trees were reexamined November 3, 1890. At that time only 

 32 of the 56 stocks bore peach shoots. Upon the rest the buds failed 

 to take or died soon after commencing to grow. In 30 of the stocks 

 the buds had grown into shoots, which were 15 to 30 inches long. In 

 many cases both buds grew. The foliage, which had not yet fallen, was 

 normal, except for slight parasitism of Puccinia pruni, Pers., and the 

 shoots were vigorous. There were no rosettes, no winter buds were 

 germinating, nor were there any other signs of the disease. One bud 

 only developed in each of the other two. One had grown only about 8 

 inches, but was normal j the other had grown only -J inch and the foli- 

 age was reddish and unhealthy. All of the stocks appeared to be as 

 healthy as when the buds were inserted. 



The parent tree was also examined at this time. The disease had 

 made considerable progress, but careful search failed to discover any 

 germinating buds or sickly shoots upon that part of the tree which 

 supplied the buds for inoculation. There were signs of disease at the 

 union of the main limb and the stem, but none further up. All large 

 parts of the tree were still alive. 



This tree was reexamined June 8, 1891. It bore no normal leaves 

 or shoot-axes. The limbs which were first to manifest disease in 1890 

 were now dead in great part, and all other portions of the tree were 

 badly diseased, including the branches from which the buds were taken. 

 Unfortunately the extreme ends of the branches were dead without 

 symptoms of rosette or foliage of any sort, but they were living last 

 November and are now alive to within 1 foot of the cuts, and bear 

 very sickly rosettes. 



The peach tops inoculated upon the Mariana plums were again exam- 

 ined June 22, 1891, and the contrast between them and the condition 

 of the tree which furnished the buds was very great. They looked as 

 healthy and vigorous as did the north side of the parent tree when the 

 buds were cut. 



Fifteen stocks bore double shoots and 16 bore single shoots, making 

 a total of 46 living peach shoots on 31 stocks. A very few of the 

 remaining Mariana stocks died; the rest grew thriftily and were cut 

 back to the earth at the time of this examination. None developed any 

 symptoms of rosette. The average growth of the peach shoots to 

 date is 3 to 3J feet, and their diameter at the base where they join the 

 plum is f to f inch. They show no symptoms of rosette or decline, 

 while the parent tree is now affected in all parts and already half dead. 



