18 as there were white corpuscles in the entire circulating blood of 

 the animal. Inasmuch as many corpuscles had been found in almost 

 every field of most of the blood-preparations of this investigation, it 

 was expected that many bacteria would be found in each field of the 

 slides of the steer's blood. No. 18 was bled from the jugular vein, 

 of the side which had not received the inoculation, at intervals of 

 thirty minutes, five hours, eleven hours, twenty-five hours, forty-six 

 hours and ten days from inoculation. The routine miscroscopic 

 blood examination failed to reveal any tubercle bacilli. 



Part of a mixture of the blood sediments of the first five bleedings 

 was inoculated intraperitoneally into a guinea pig, which was 

 allowed to live eleven weeks. Another guinea pig received the sedi- 

 ment of the sixth and last bleeding and was killed at the end of ten 

 weeks. At autopsy both animals appeared to be entirely normal. 



The steer's temperature rose rapidly to 105.5 after inoculation, but 

 fell to normal the next morning. After a few days the temperature 

 began to rise again, and four weeks after inoculation the animal died. 

 Autopsy, performed twenty-four hours after death, showed the lungs 

 to be crowded with miliary tubercles. The spleen contained on the 

 average one miliary tubercle in each square centimeter of its surface. 

 The kidneys contained a few miliary tubercles and the liver none. 

 The mediastinal, inguinal, pelvic, and crural lymph-nodes were en- 

 larged and soft. In the heart were a clot and some red turbid serum. 

 The centrifugalized sediment of this serum showed many coarse 

 bacilli and one group of three tubercle bacilli. 



Cow 7 was bled one hour, five hours, eleven hours, twenty-five 

 hours, forty-six hours and fourteen days after inoculation, and the 

 blood was examined. One short structure resembling a tubercle 

 bacillus was found in a smear from the top layer of the sediment 

 drawn eleven hours after inoculation. This slide had been decol- 

 orized by the rosolic acid solution. All the other slides were unques- 

 tionably negative. 



A guinea pig which received part of a mixture of the sediment 

 from the first five of the six bleedings was killed eleven weeks after 

 inoculation and found to be normal. 



The cow's temperature rose immediately after inoculation to 107.5, 

 and in a few hours fell almost to normal. Then the temperature rose 

 gradually to 107.8 and subsided almost to normal. The curve of this 

 last rise and fall suggested the course of a severe tuberculin reaction. 

 No corresponding rise and fall had occurred in the temperature of 

 steer 18. The cow died nine weeks after inoculation. Autops> 

 showed extensive chronic lesions and no acute miliary tuberculosis. 

 The right submaxillary, bronchial, anterior mediastinal, portal and 

 mesenteric lymph-nodes were found calcified. The left mediastinal 

 and parotid lymph-nodes were caseous. The left submaxillary 

 lymph-nodes had broken down and had formed a large abscess. 

 Both lungs were extensively involved with old lesions, some con- 

 necting with the bronchi. The spleen showed a calcified nodule 2 

 cm. in diameter. The liver and kidneys were normal. 



A third time we introduced tubercle bacilli into the circulation of 

 the cattle and attempted to recover them from their blood. Cow 3 

 of Group A had advanced tuberculosis. Tubercle bacilli had been 

 found in the pus of a large axillary abscess. The animal was put 



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