X CONTENTS 



PAGE 

 XII. SPECIAL MEDIA 182 



XIII. INCUBATORS 216 



XIV. METHODS OF CULTIVATION . . . 221 



Aerobic, 222 Anaerobic, 236. 



XV. METHODS OF ISOLATION 248 



XVI. METHODS OF IDENTIFICATION AND STUDY 259 



Scheme of Study, 259 Macroscopical Examination 

 of Cultivations, 261 Microscopical Methods, 272 

 Biochemical Methods, 276 Physical Methods, 295 

 Inoculation Methods, 315 Immunisation, 321 

 Active Immunisation, 322 The Preparation of 

 Haemolytic Serum, 327 The Titrationof Haemolytic 

 Serum, 328 Storage of Haemolysin, 331. 



XVII. EXPERIMENTAL INOCULATION OF ANIMALS ...... 332 



Selection and Care of Animals, 335 Methods of 

 Inoculation, 352. 



XVIII. THE STUDY OF EXPERIMENTAL INFECTIONS DURING LIFE 370 



General Observations, 371 Blood Examinations, 

 373 Serological Investigations, 378 Agglutinin, 

 381 Opsonin, 387 Immune Body, 393. 



XIX. POST-MORTEM EXAMINATION OF EXPERIMENTAL ANIMALS 396 

 XX. THE STUDY OF THE PATHOGENIC BACTERIA 408 



XXI. BACTERIOLOGICAL ANALYSES 415 



Bacteriological Examination of Water, 416 Ex- 

 amination of Milk, 441 Ice Cream, 457 Ex- 

 amination of Cream and Butter, 457 Examination 

 of Unsound Meats, 460 Examination of Oysters 

 and Other Shellfish, 463 Examination of Sewage 

 and Sewage Effluents, 466 Examination of Air, 468 

 Examination of Soil, 470 Testing Filters, 478 

 Testing of Disinfectants, 480. 



APPENDIX 492 



INDEX .505 



