CONTENTS 



PAGE 

 I. LABORATORY REGULATIONS i 



II. GLASS APPARATUS IN COMMON USE ' . . 3 



The Selection, Preparation, and Care of Glassware, 

 8 Cleaning of Glass Apparatus, 18 Plugging 

 Test-tubes and Flasks, 24. 



III. METHODS OF STERILISATION 26 



Sterilising Agents, 26 Methods of Application, 27 

 Electric Signal Timing Clock, 38. 



IV. THE MICROSCOPE 49 



Essentials, 49 Accessories, 57 Methods of Micro- 

 metry, 61. 



V. MICROSCOPICAL EXAMINATION OF BACTERIA AND OTHER 



MICRO-FUNGI 69 



Apparatus and Reagents used in Ordinary Micro- 

 scopical Examination, 69 Methods of Examina- 

 tion, 74. 



VI. STAINING METHODS 90 



Bacteria Stains, 90 Contrast Stains, 93 Tissue 

 Stains, 95 Blood Stains, 97 Methods of Demon- 

 strating Structure of Bacteria, 99 Differential 

 Methods of Staining, 108. 



VII. METHODS OF DEMONSTRATING BACTERIA IN TISSUES 114 

 Freezing Method, 115 Paraffin Method, 117 

 Special Staining Methods for Sections, 121. 



VIII. CLASSIFICATION OF FUNGI 126 



Morphology of the Hypomycetes, 126 Morphol- 

 ogy of the Blastomycetes, 129. 



IX. SCHIZOMYCETES 13! 



Anatomy, 134 Physiology, 136 Biochemistry, 

 144. 



X. NUTRIENT MEDIA 146 



Meat Extract, 148 Standardisation of Media, 154 

 The Filtration of Media, 156 Storing Media in 

 Bulk, 159 Tubing Nutrient Media, 160. 



XI. ORDINARY OR STOCK CULTURE MEDIA 163 



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