SIMPLE STAINS 9! 



Place in a stoppered bottle of 150 c.c. capacity and 

 add 



Alcohol, 90 per cent 100.0 c.c. 



Allow the alcohol to remain in contact with the dye 

 for two hours, shaking vigourously every few minutes. 

 Filter. 



3. Carbolic Meikylene-blue (Kuehne). 

 Weigh out 



Methylene-blue ........ i.$ grammes 



Carbolic acid 5.0 grammes 



and dissolve in 



Distilled water 100.0 c.c. 



and add 



Absolute alcohol 10.0 c.c. 



Filter. 



4. Alkaline Methylene-blue (Loeffler). 

 Measure out and mix 



Methylene-blue, saturated alcoholic solution. . 30.0 c.c. 

 Caustic potash, o.oi per cent, aqueous solution . 100.0 c.c. 



Filter. 



Gentian Violet. 



5. Saturated Aqueous Solution. 

 Weigh out 



Gentian violet 2.25 grammes 



and proceed as in preparing the corresponding solution 

 of methylene-blue. 



6. Saturated Alcoholic Solution. 

 Weigh out 



Gentian violet 5.0 grammes 



and proceed as in preparing the corresponding solution 

 of methylene-blue. 



