9 2 



STAINING METHODS 



7. Carbolic Gentian Violet (Nicolle). 

 Measure out and mix 



Gentian violet, saturated alcoholic solution . 10.0 c.c. 

 Carbolic acid, i per cent, aqueous solution . 100.0 c.c. 



Filter. 



8. Anilin Water Solution (Koch-Ehrlich) . 

 Measure out 



Distilled water 100 c.c. 



Add anilin oil drop by drop (shaking well after the 

 addition of each drop) until the solution is opaque. 



Filter until clear, 

 and add 



Absolute alcohol 10 c.c. 



Saturated alcoholic solution gentian violet . n c.c. 



Filter. 



NOTE. This solution will not keep longer than 14 days. 



Thionine Blue (or Lauth's Violet) . 



9. Carbolic Thionine Bine (Nicolle). 

 Weigh out 



Thionine blue i . o gramme 



Carbolic acid 2.5 grammes 



and dissolve in 



Distilled water 100.0 c.c. 



Filter. 



Before use dilute with equal quantity of distilled 

 water and again filter. 



Fuchsin (Basic). 



10. Saturated Aqueous Solution. 

 Weigh out 



Basic fuchsin 1.5 grammes 



and proceed as in preparing the corresponding solution 

 of methylene-blue (q. v.). 



