CONTRAST STAINS 93 



11. Saturated Alcoholic Solution. 

 Weigh out 



Basic fuchsin .3.5 grammes 



and proceed as in preparing the corresponding solu- 

 tion of methylene-blue. 



1 2 . Carbolic Fuchsin (Ziehl) . 

 Weigh out 



Basic fuchsin i . o gramme 



Carbolic acid 5.0 grammes 



dissolve in 



Distilled water 100.0 c.c. 



and add 



Absolute alcohol 10.0 c.c. 



Filter. 



CONTRAST STAINS. 



Eosin. There are several commercial varieties of 

 eosin, which, from the bacteriological point of view, 

 possess very different values. Gruebler lists four varie- 

 ties, of which two only are useful for bacteriological 

 work: 



Eosin, aqueous yellowish. 



Eosin, aqueous bluish. 



13. Eosin Aqueous Solution (Yellowish or Bluish 

 Shade), i per cent. 



Weigh out 



Eosin, aqueous i.o gramme 



dissolve in 



Distilled water 100.0 c.c. 



and add 



Absolute alcohol 5.0 c.c. 



Filter. 



