H^EMATIN 



95 



TISSUE STAINS. 



Aniline Gentian Violet (For Weigert's Fibrin Stain) . 

 Weigh out 



Gentian violet . i . o gramme 



and dissolve in 



Absolute alcohol I 5- c -c. 



Distilled water 80.0 c.c. 



then add 



Aniline oil 3.0 c.c. 



Shake well and filter before use. 



Haematoxylin (Ehrlich). 



1. Weigh out 



Haematoxylin ..2.0 grammes 



and dissolve in 



Absolute alcohol 100.0 c.c. 



2. Weigh out 



Ammonium alum 2.0 grammes 



and dissolve in 



Distilled water loo.oc.c. 



3. Mix i and 2, allow the mixture to stand forty- 

 ight hours, then filter. 



4. Add 



Glycerine 85.0 c.c. 



Acetic acid, glacial ic.oc.c. 



5. Allow the stain to stand for one month exposed 

 to light ; then filter again ready for use. 



Hsematin (Mayer's). 

 A. Weigh out 



Haematin i . o gramme 



and dissolve in 



Alcohol 90 per cent, (warmed to 37 C.) . 50 c.c. 



