96 STAINING METHODS 



B. Weigh out 



Potash alum 50 grammes 



and dissolve in 



Distilled water 100 c.c. 



Prepare these two solutions in separate flasks. 

 Take a clean flask of 250 c.c. capacity and insert a large 

 funnel in its neck. Pour the solutions A and B simul- 

 taneously and slowly into the funnel to mix thoroughly. 

 Store for future use. 



NOTE. If acid haematin is required, introduce glacial acetic 

 acid (3 c.c.) into the mixing flask before adding the solutions A 

 and B. 



Alum Carmine (Mayer) . 

 Weigh out 



Alum 2.5 grammes 



Carmine i . o gramme 



and place in a glass beaker. 

 Measure out in a measuring cylinder, 



Distilled water 100.0 c.c. 



Place the beaker on a sand-bath, add the water in 

 successive small quantities, and keep the mixture boil- 

 ing for twenty minutes. Measure the solution and 

 make up to 100 c.c. by the addition of distilled water. 

 Filter. 



Lithium Carmine (Orth). 

 Weigh out 



Carmine 2.5 grammes 



and dissolve in 



Lithium carbonate, cold saturated solution . 100.0 c.c. 



Filter. 

 Picrocarmine. 



Weigh out 



Picrocarmine 2.0 grammes 



