122 



DEMONSTRATING BACTERIA IN TISSUES 



4. Wash in water. 



5. Wash in alcohol. 



6. Stain in aniline gentian violet. 



7. Wash in iodine solution till dark brown or black. 



8. Wash in water. 



9. Dip in absolute alcohol for a second. 



10. Decolourise with aniline oil till no more colour 

 is discharged. 



FIG. 76. Staining pot. 



11. Wash with aniline oil, 2 parts, xylol, i part. 



12. Clear with xylol. 



13. Mount in xylol balsam. 



Alternative Gram=Weigert Method for Sections. 



1. Fix paraffin section on slide and prepare for stain- 

 ing in the usual manner. 



2. Stain in alum carmine for about fifteen minutes. 



3 . Wash thoroughly in water. 



4. Filter aniline gentian violet solution on to the 

 section on the slide and allow to stain about twenty- 

 five minutes. 



5. Wash thoroughly in water. 



6. Treat with Lugol's iodine until section ceases to 

 become any blacker. 



7. Wash thoroughly in water. 



8. Treat with a mixture of equal parts of aniline 

 oil and xylol until no more colour comes away. 



